Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CIDEC Primary Antibodies »

Product Specifications

Immunogen

Residues 10-25 LLYPKSLSRHVSVRTS] of the 27 kDa human FSP27 (CIDE-3) protein.

Localization

Cytoplasmic

Specificity

This is specific for CIDE-3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

26.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit CIDEC Antibody - BSA Free (NB100-430) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-CIDEC Antibody: Cited in 16 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for CIDEC Antibody - BSA Free

Western Blot: CIDEC AntibodyBSA Free [NB100-430]

Western Blot: CIDEC AntibodyBSA Free [NB100-430]

Western Blot: CIDEC Antibody [NB100-430] - Analysis of CIDEC using NB100-430 at 1:1000 dilution on 30ug HepG2 cell lysate.

Applications for CIDEC Antibody - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:1000
Application Notes
This antibody may be used for Western blot.Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23466592) No other assays have been tested. Suggested working dilutions: * Western Blot - 1:500 ~ 1:1,000 *The investigator should determine the optimal working dilution for a specific application. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 5 using NB100-430 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CIDEC

DNA fragmentation is one of the critical steps in apoptosis, which is induced by DNA fragmentation factor (DFF). DFF is composed of two subunits, a 40 kDa caspase-activated nuclease (DFF40) and a 45 kDa inhibitor (DFF45). Recently a novel family of cell-death-inducing DFF45-like effectors (CIDEs) has been identified. Human CIDE-3 is a novel member of CIDEs. There are 2 transcripts, CIDE-3 and CIDE-3alpha, were present in HepG2 and A375 cells. Consistent with its chromosome localization at 3p25, a region associated with high frequency loss of heterozygosity in many tumors, CIDE-3 may play an important role in prevention of tumorigenesis.

Long Name

Cell Death-inducing DFFA-like Effector C

Alternate Names

CIDE-3, CIDEX, FSP27

Entrez Gene IDs

63924 (Human); 14311 (Mouse)

Gene Symbol

CIDEC

UniProt

Q96AQ7 (Human)

Additional CIDEC Products

Product Documents for CIDEC Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CIDEC Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CIDEC Antibody - BSA Free

Customer Reviews for CIDEC Antibody - BSA Free (1)

5 out of 5
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 19258494
    Species: Mouse
    Verified Customer | Posted 12/12/2014

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