Cingulin Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15904

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cingulin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human Cingulin. The exact sequence is proprietary.

Reactivity Notes

Mouse (86%), Rabbit (83%), Bovine (85%).

Localization

Cell junction; tight junction

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

136 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cingulin Antibody - BSA Free

Western Blot: Cingulin Antibody [NBP2-15904]

Western Blot: Cingulin Antibody [NBP2-15904]

Western Blot: Cingulin Antibody [NBP2-15904] - Sample (30 ug of whole cell lysate) A: Juakat 7. 5% SDS PAGE gel, diluted at 1:500.
Immunohistochemistry-Paraffin: Cingulin Antibody [NBP2-15904]

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP2-15904]

Immunohistochemistry-Paraffin: Cingulin Antibody [NBP2-15904] - Cytoplasm on Ovarian carcinoma; antibody dilution: 1:500.
Cingulin Antibody

Immunocytochemistry/ Immunofluorescence: Cingulin Antibody [NBP2-15904] -

Immunocytochemistry/ Immunofluorescence: Cingulin Antibody [NBP2-15904] - Cingulin antibody detects Cingulin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Cingulin stained by Cingulin antibody (NBP2-15904) diluted at 1:500.
Cingulin Antibody

Western Blot: Cingulin Antibody [NBP2-15904] -

Western Blot: Cingulin Antibody [NBP2-15904] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with Cingulin antibody (NBP2-15904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Cingulin Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cingulin

Probably plays a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier.

Alternate Names

cingulin, DKFZp779N1112, KIAA1319

Gene Symbol

CGN

Additional Cingulin Products

Product Documents for Cingulin Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cingulin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

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FAQs

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