Cited-2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-24743

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cited-2 Primary Antibodies »

Product Specifications

Immunogen

This antibody has been engineered to specifically recognize the recombinant protein Cited-2 using the following amino acid sequence: SGSGNMPASVAHVPAAMLPPNVIDTDFIDEEVLMS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cited-2 Antibody - BSA Free

Cited-2 Antibody Western Blot: Cited-2 Antibody [NBP3-24743]

Western Blot: Cited-2 Antibody [NBP3-24743]

Analysis in human cell line RT-4 and human cell line U-251 MG.
Cited-2 Antibody Immunocytochemistry/Immunofluorescence: Cited-2 Antibody [NBP3-24743]

Immunocytochemistry/Immunofluorescence: Cited-2 Antibody [NBP3-24743]

Staining of human cell line HEL shows localization to nucleoplasm & cytosol.

Applications for Cited-2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Western Blot

0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Cited-2

CITED2 is a cAMP-responsive element-binding protein (CBP)/p300 interacting transcriptional modulator. CITED-2 has been proposed as a negative regulator for HIF-1 alpha, as it is thought to interfere with the binding of transcription factors HIF-1a and STAT2 to p300/CBP. Recruitment of CBP/p300 by HIF-1 is essential for the transcriptional response to hypoxia.

CITED 2 antibodies are useful tools for research on hypoxic response mechanisms.

Long Name

Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2

Alternate Names

Cited2, MRG-1, MSG-related protein 1, P35srj

Gene Symbol

CITED2

Additional Cited-2 Products

Product Documents for Cited-2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Cited-2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

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Associated Pathways

HIF Repressor Pathways HIF Repressor Pathway Thumbnail