CKAP4/p63 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-85571

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CKAP4/p63 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LKDLSDGIHVVKDARERDFTSLENTVEERLTELTKSINDNIAIFTEVQKRSQKEINDMKAKVASLEESEGNKQDLKALKEAVKEIQTSAKSREWDMEALRSTLQTMES

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CKAP4/p63 Antibody - BSA Free

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining in human cervix, uterine and skeletal muscle tissues using anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human bone marrow, cerebral cortex, cervix, uterine and skeletal muscle using Anti-CKAP4 antibody NBP1-85571 (A) shows similar protein distribution across tissues to independent antibody NBP1-85572 (B).
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human skeletal muscle using Anti-CKAP4 antibody NBP1-85571.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human cervix, uterine shows high expression.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human bone marrow using Anti-CKAP4 antibody.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human cerebral cortex using Anti-CKAP4 antibody.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP1-85571] - Staining of human cervix, uterine using Anti-CKAP4 antibody NBP1-85571.
CKAP4/p63 Antibody - BSA Free Immunohistochemistry-Paraffin: CKAP4/p63 Antibody - BSA Free [NBP1-85571]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody - BSA Free [NBP1-85571]

Staining of human skeletal muscle shows low expression as expected.
CKAP4/p63 Antibody - BSA Free Western Blot: CKAP4/p63 Antibody - BSA Free [NBP1-85571]

Western Blot: CKAP4/p63 Antibody - BSA Free [NBP1-85571]

Analysis in mouse cell line NIH-3T3, rat cell line NBT-II and rat cell line pC12.
CKAP4/p63 Antibody - BSA Free Western Blot: CKAP4/p63 Antibody - BSA Free [NBP1-85571]

Western Blot: CKAP4/p63 Antibody - BSA Free [NBP1-85571]

Analysis in human cell lines U-251MG and MCF-7 using Anti-CKAP4 antibody. Corresponding CKAP4 RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.

Applications for CKAP4/p63 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500-1:1000

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CKAP4/p63

Cytoskeleton-associated protein 4 (CKAP4) is also known as p63 and cytoskeleton-linking membrane protein 63kDa (CLIMP-63). It was first described as a palmitoylated single-pass transmembrane receptor that localized to the rough endoplasmic reticulum as well as the cell membrane. CKAP4 has been shown to help anchor endoplasmic reticulum to microtubules.

Long Name

Cytoskeleton-associated Protein-4/Climp-63

Alternate Names

CLIMP-63, ERGIC-63, p63

Gene Symbol

CKAP4

Additional CKAP4/p63 Products

Product Documents for CKAP4/p63 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for CKAP4/p63 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Cancer Biomarkers

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Protocols

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