CKAP4/p63 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-15908

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CKAP4/p63 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human CKAP4/p63. The exact sequence is proprietary.

Localization

Endoplasmic reticulum-Golgi intermediate compartment membrane

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

66 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CKAP4/p63 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CKAP4/p63 Antibody [NBP2-15908]

Immunocytochemistry/ Immunofluorescence: CKAP4/p63 Antibody [NBP2-15908]

Immunocytochemistry/Immunofluorescence: CKAP4/p63 Antibody [NBP2-15908] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: CKAP4 stained by CKAP4 antibody [N3C3] diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000. Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - Human colon cancer. CKAP4 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908]

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CL1-5 xenograft, using CKAP4 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
CKAP4/p63 Antibody

Western Blot: CKAP4/p63 Antibody [NBP2-15908] -

Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
CKAP4/p63 Antibody

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] -

Immunohistochemistry-Paraffin: CKAP4/p63 Antibody [NBP2-15908] - CKAP4/p63 antibody [N3C3] detects CKAP4/p63 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
CKAP4/p63 stained by CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
CKAP4/p63 Antibody

Western Blot: CKAP4/p63 Antibody [NBP2-15908] -

Western Blot: CKAP4/p63 Antibody [NBP2-15908] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CKAP4/p63 antibody [N3C3] (NBP2-15908) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for CKAP4/p63 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:5000-1:20000
Application Notes
IP- Assay dependent.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: CKAP4/p63

Cytoskeleton-associated protein 4 (CKAP4) is also known as p63 and cytoskeleton-linking membrane protein 63kDa (CLIMP-63). It was first described as a palmitoylated single-pass transmembrane receptor that localized to the rough endoplasmic reticulum as well as the cell membrane. CKAP4 has been shown to help anchor endoplasmic reticulum to microtubules.

Long Name

Cytoskeleton-associated Protein-4/Climp-63

Alternate Names

CLIMP-63, ERGIC-63, p63

Gene Symbol

CKAP4

UniProt

Q07065 (Human)

Additional CKAP4/p63 Products

Product Documents for CKAP4/p63 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for CKAP4/p63 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers

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Protocols

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FAQs

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