CKII alpha prime polypeptide Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-35650

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CKII alpha prime polypeptide Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human CKII alpha prime polypeptide (NP_001887.1).

Sequence:
LGTEELYGYLKKYHIDLDPHFNDILGQHSRKRWENFIHSENRHLVSPEALDLLDKLLRYDHQQRLTAKEAMEHPYFYPVVKEQSQPCADNAVLSSGLTAAR

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

41 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CKII alpha prime polypeptide Antibody - BSA Free

CKII alpha prime polypeptide Antibody

Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] -

Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of various lysates using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
CKII alpha prime polypeptide Antibody

Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] -

Western Blot: CKII alpha prime polypeptide Antibody [NBP3-35650] - Western blot analysis of lysates from HeLa cells, using CKII alpha prime polypeptide Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s.
CKII alpha prime polypeptide Antibody

Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] -

Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] - Immunofluorescence analysis of NIH/3T3 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
CKII alpha prime polypeptide Antibody

Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] -

Immunocytochemistry/ Immunofluorescence: CKII alpha prime polypeptide Antibody [NBP3-35650] - Immunofluorescence analysis of PC-12 cells using CKII alpha prime polypeptide Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for CKII alpha prime polypeptide Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CKII alpha prime polypeptide

CKII alpha is involved in leukemogensis and tumorigenesis. It is a catalytic, ubiquitous cellular kinase that can use ATP and GTP as phosphorylation donors. CKII alpha also plays a role in many proliferation-related processes in the cell.

Long Name

Casein kinase II subunit alpha'

Alternate Names

CK II alpha', CK2A2, CSNK2A2

Gene Symbol

CSNK2A2

Additional CKII alpha prime polypeptide Products

Product Documents for CKII alpha prime polypeptide Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CKII alpha prime polypeptide Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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