Claudin-11 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-82470

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Chicken

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Claudin-11 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DAQAFGENRFYYTAGSSSPTHAKSAHV

Reactivity Notes

Rat (89%). Mouse reactivity reported in scientific literature (PMID: 25630681). Chicken reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Claudin-11 Antibody - BSA Free

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470] - Analysis in human testis and pancreas tissues using NBP1-82470 antibody. Corresponding CLDN11 RNA-seq data are presented for the same tissues.
Western Blot: Claudin-11 Antibody [NBP1-82470]

Western Blot: Claudin-11 Antibody [NBP1-82470]

Western Blot: Claudin-11 Antibody [NBP1-82470] - HUVEC (Human Umbilical Vein Endothelial Cells) lysate. Three dilutions were tested using the WES 12-230 kDa kit (left panel): Lanes 2-3, 1:100; Lanes 4-5, 1:50; and Lanes 6-7, 1:25. The 1:25 dilution was tested again using the 2-40 kDa (right panel). All test lanes utilized 0.2 mg/mL protein concentrations. The WES anti-rabbit antibody kit was used. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470] - Staining of human Skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470] - Staining of human Cervix shows no positivity in squamous epithelial cells as expected.
Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470] - Staining of human Pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470]

Immunohistochemistry-Paraffin: Claudin-11 Antibody [NBP1-82470] - Staining of human Testis shows strong membranous positivity in cells in seminiferous ducts.
Claudin-11 Antibody

Western Blot: Claudin-11 Antibody [NBP1-82470] -

Western Blot: Claudin-11 Antibody [NBP1-82470] - Comparison of expression changes between young & old MRC-5 & HFF fibroblasts measured with RNA-seq & Western Blots. (a) 8 genes commonly downregulated & (b) 8 genes commonly upregulated in both cell lines. (a, b) The colors of the bars indicate the measurement technique (blue: RNA-seq; red: Western Blots/protein expression). Solid colored bars represent MRC-5 while shaded boxes represent HFF cells. The height of the bars corresponds to the logarithmic fold-change (FC) of expression between the first & the last PD investigated here (RNA-seq: log 2 RPKM FC; protein: log 2 expression ratio). Error bars indicate standard deviation from the mean. Changes statistically different comparing young & old PD (RNA-seq: DESeq; rRT-PCR/Protein: Student's t-test; n = 3) are indicated with an asterix: ∗p < 0.05, ∗∗p < 0.01, & ∗∗∗p < 0.001. (c) The blots show the protein expression levels in MRC-5 & HFF cells at young compared to old PDs. The up- or downregulation was signified by the presence or absence of bands in Western Blots. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/26339636), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Claudin-11 Antibody - BSA Free

Immunohistochemistry: Rabbit Polyclonal Claudin-11 Antibody [NBP1-82470]

Analysis of chicken embryo spinal cord at E9.5. Cryosections were stained using a primary antibody dilution of 1:100.
White: DAPI
Red: TUBB3
Cyan: Claudin 11
Image from a verified customer review.

Applications for Claudin-11 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP1-82470 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Claudin-11

Oligodendrocyte specific protein belongs is a major component of central nervous system myelin that is necessary for normal CNS function. The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, and are located in both epithelial and endothelial cells in all tight junction bearing tissues. Claudins, which consist of four transmembrane domains and two extracellular loops, make up tight junction strands. There is growing evidence that the Claudin 11 protein determines the permeability between layers of myelin sheaths via focal adhesion and with its expression highly regulated during development, may play an important role in cellular proliferation and migration. In addition, the protein is a candidate autoantigen in the development of autoimmune demyelinating disease.

Alternate Names

Claudin11, CLDN11, OSP, OTM

Gene Symbol

CLDN11

Additional Claudin-11 Products

Product Documents for Claudin-11 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Claudin-11 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Claudin-11 Antibody - BSA Free

Customer Reviews for Claudin-11 Antibody - BSA Free (2)

4.5 out of 5
2 Customer Ratings
5 Stars
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4 Stars
50%
3 Stars
0%
2 Stars
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1 Stars
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Customer Images

Showing  1 - 2 of 2 reviews Showing All
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  • Claudin11 Chicken and Mouse Embryo
    Name: Gauthier Toulouse
    Application: Immunofluorescence
    Sample Tested: Embryos
    Species: Chicken and Mouse
    Verified Customer | Posted 03/19/2026
    White: DAPI Red: TUBB3 Cyan: Claudin 11 Spinal cord E9.5 chicken embryo
    1/100 on cryosections
    Claudin-11 Antibody - BSA Free NBP1-82470
    Bio-Techne Response
    This review reflects a new species or application tested on a primary antibody.
  • Claudin-11 Antibody
    Name: Jerome Breslin
    Application: Simple Western
    Sample Tested: HUVEC (Human Umbilical Vein Endothelial Cells)
    Species: Human
    Verified Customer | Posted 06/07/2021
    Three dilutions were tested using the WES 12-230 kDa kit (left panel): Lanes 2-3, 1:100; Lanes 4-5, 1:50; and Lanes 6-7, 1:25. The 1:25 dilution was tested again using the 2-40 kDa (right panel).
    All test lanes utilized 0.2 mg/ml protein concentrations. The WES anti-rabbit antibody kit was used.
    Claudin-11 Antibody - BSA Free NBP1-82470

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