CLEC10A/CD301 Antibody (CL15513) - BSA Free

Novus Biologicals | Catalog # NBP3-43672

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL15513

Format

BSA Free
View all CLEC10A/CD301 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of Q8IUN9, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CLEC10A/CD301 Antibody (CL15513) - BSA Free

CLEC10A/CD301 Antibody (CL15513) [Unconjugated] Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Staining of human placenta shows no positivity in trophoblastic cells as expected.
CLEC10A/CD301 Antibody (CL15513) [Unconjugated] Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Staining of human cerebral cortex shows moderate membranous-cytoplasmic positivity in endothelial cells and neuropil.
CLEC10A/CD301 Antibody (CL15513) [Unconjugated] Western blot: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Western blot: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Analysis in control (vector only transfected HEK293T lysate) and CLEC10A over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
CLEC10A/CD301 Antibody (CL15513) [Unconjugated] Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Staining of human rectum shows strong membranous-cytoplasmic positivity in lymphoid cells.
CLEC10A/CD301 Antibody (CL15513) [Unconjugated] Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Analysis in human tonsil and placenta tissues using NBP3-43672 antibody. Corresponding CLEC10A RNA-seq data are presented for the same tissues.
CLEC10A/CD301 Antibody (CL15513) [Unconjugated] Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Immunohistochemistry-Paraffin: CLEC10A/CD301 Antibody (CL15513) [NBP3-43672]

Staining of human tonsil shows strong membranous-cytoplasmic positivity in non-germinal center cells.

Applications for CLEC10A/CD301 Antibody (CL15513) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS, pH 7.2, 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLEC10A/CD301

CD301 encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. The encoded type 2 transmembrane protein may function as a cell surface antigen. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Macrophage Galactose N-Acetyl-Galactosamine Specific Lectin 1

Alternate Names

CD301, CLECSF14, HML2, MGL

Gene Symbol

CLEC10A

Additional CLEC10A/CD301 Products

Product Documents for CLEC10A/CD301 Antibody (CL15513) - BSA Free

Certificate of Analysis

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Product Specific Notices for CLEC10A/CD301 Antibody (CL15513) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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