CLEC4E Antibody (OTI2A8) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-71793
![Western Blot: CLEC4E Antibody (OTI2A8)Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Western-Blot-NBP2-71793-img0001.jpg "Western Blot: CLEC4E Antibody (OTI2A8)Azide and BSA Free [NBP2-71793]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI2A8
Format
Azide and BSA Free
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Product Specifications
Immunogen
Full length human recombinant protein of human CLEC4E(NP_055173) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
24.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Mouse CLEC4E Antibody (OTI2A8) - Azide and BSA Free (NBP2-45629) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for CLEC4E Antibody (OTI2A8) - Azide and BSA Free
Western Blot: CLEC4E Antibody (OTI2A8)Azide and BSA Free [NBP2-71793]
Western Blot: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CLEC4E.![Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71793-img0008.jpg "Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]")
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of Human Ovary tissue.![Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71793-img0003.jpg "Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]")
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of Adenocarcinoma of Human endometrium tissue.![Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71793-img0004.jpg "Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]")
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of Adenocarcinoma of Human ovary tissue.![Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71793-img0005.jpg "Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]")
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of Carcinoma of Human prostate tissue.![Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71793-img0006.jpg "Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]")
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of Human Kidney tissue.![Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71793-img0007.jpg "Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]")
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of Human lymphoma tissue.![Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]](https://beta-resources.rndsystems.com/images/products/CLEC4E-Antibody-OTI2A8-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71793-img0009.jpg "Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]")
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793]
Immunohistochemistry: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of Human pancreas tissue.
CyTOF-ready: CLEC4E Antibody (OTI2A8) - Azide and BSA Free [NBP2-71793] - Analysis of HEK293T cells transfected with either RC200244 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostaining by CLEC4E antibody, and then analyzed by flow cytometry.
Applications for CLEC4E Antibody (OTI2A8) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunohistochemistry
1:150
Western Blot
1:2000
Flow Cytometry Panel Builder
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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.
Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Calculators
Background: CLEC4E
Long Name
C-type Lectin Domain Family 4, Member E
Alternate Names
CLECSF9, MINCLE
Gene Symbol
CLEC4E
Additional CLEC4E Products
Product Documents for CLEC4E Antibody (OTI2A8) - Azide and BSA Free
Product Specific Notices for CLEC4E Antibody (OTI2A8) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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