CLOCK Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-88614

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CLOCK Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: TQDRQIRFSQGQQLVTKLVTAPVACGAVMVPSTMLMGQVVTAYPTFATQQQQSQTLSVTQQQQQQSSQEQQLTSVQQPSQAQLTQPPQQFLQTSRLLHGNPSTQLILSAAFPLQQSTFPQSHHQQHQSQQQQQLSRHRTDSLPD

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CLOCK Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody [NBP1-88614]

Immunocytochemistry/ Immunofluorescence: CLOCK Antibody [NBP1-88614]

Immunocytochemistry/Immunofluorescence: CLOCK Antibody [NBP1-88614] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614]

Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614]

Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] - Staining of human testis shows moderate nuclear positivity in cells in seminiferous ducts.
CLOCK Antibody

Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] -

Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] -Staining of human duodenum shows strong nuclear and cytoplasmic positivity in glandular cells.
CLOCK Antibody

Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] -

Immunohistochemistry-Paraffin: CLOCK Antibody [NBP1-88614] -Staining of human kidney shows moderate nuclear and cytoplasmic positivity in cells in tubules.
CLOCK Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: CLOCK Antibody - BSA Free [NBP1-88614]

Chromatin Immunoprecipitation-exo-Seq: CLOCK Antibody - BSA Free [NBP1-88614]

ChIP-Exo-Seq composite graph for Anti-CLOCK (NBP1-88614) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for CLOCK Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CLOCK

Circadian Locomotor Output Cycles Kaput (CLOCK) is a bHLH/PAS protein that plays an important role in the molecular clock mechanism. Specifically, CLOCK activates the central circadian loop by stimulating expression of Period and Timeless proteins. CLOCK has also been shown to interact strongly with other bHLH-PAS proteins such as MOP3, a BMAL1 isoform.

CLOCK levels peak during the late night and the early part of the subjective day. Mutations in CLOCK causes defective transcriptional activity and lengthens the circadian period, leading to abnormal circadian behavior.

CLOCK antibodies are useful tools for research on circadian rythmns, sleep biology and hormonal cycles.

Long Name

Circadian Locomoter Output Cycles Kaput

Alternate Names

BHLHE8, circadian locomoter output cycles protein kaput, Class E basic helix-loop-helix protein 8, clock (mouse) homolog, clock homolog (mouse), EC 2.3.1.48, hCLOCK, KAT13D, KIAA0334bHLHe8circadian locomoter output cycles kaput protein

Entrez Gene IDs

9575 (Human)

Gene Symbol

CLOCK

UniProt

O15516

Additional CLOCK Products

Product Documents for CLOCK Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CLOCK Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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