Cloudz Human T Cell Activation Kit

Dissolvable microspheres for the expansion of T cells
Catalog # Availability Size / Price Qty
CLD001
Product Details
FAQs
Reviews

Cloudz Human T Cell Activation Kit Summary

Dissolvable, magnetic-free Cloudz CD3/CD28 microspheres for the expansion of Human T-Cells

Key Benefits

• > 50-fold expansion of T cells from PBMCs cells in 10 days
• Dissolvable CD3/28 microspheres enable T cell expansions
• Optimized for use with the ExCellerate™ Human T Cell Media
• Supports T cell expansion from PBMCs or purified CD3+ PBMCs
• Optimized for use with R&D Systems cytokines and media

Why Expand T Cells ex vivo Using Cloudz Human T Cell Activation Kit?

T Lymphocytes (T Cells) can be used as a powerful immunotherapy to fight against cancer and other diseases. Cloudz Human T cell Activation Kit allows for a robust expansion platform utilizing dissolvable CD3/28 microspheres that can easily be removed from expanded cells. Cloudz Human T Cell Activation Kit supports the expansion of human T cell lines or T cell clones and the stimulation of human peripheral blood lymphocytes.

Versatility of Cloudz Human T Cell Activation Kit

Cloudz Human T Cell Activation Kit is optimized for T cell expansion when used in combination with ExCellerate Human T cell Expansion Media, Xeno-Free and cytokines including IL-2, IL-7, and IL-15. The activation and cytokine/growth factor combinations used with this kit should be optimized by application or experimental protocol.

Kit Contents

• 12 mL of Cloudz CD3/CD28
• 100 mL of Cloudz 6X Release Buffer
Each kit contains enough reagents to activate 2x108 CD3+ T Cells.

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the recommended temperature.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Human

Limitations

For research use only. Not for diagnositic use.

Product Datasheets

FAQs

  1. Cloudz Human T Cell Activation Kit, Catalog #  CLD001, and the Cloudz Human Treg Expansion kit, Catalog # CLD006, both contain Cloudz CD3/CD28 antibodies. Can the antibodies in CLD001 be used in the CLD006 kit for Treg expansion?

    • While both kits have the similar antibodies, each product is manufactured and optimized to each type of cell expansion (T cell or Treg).  Use of Cloudz CD3/CD28 from CLD001 in CLD006 is not supported. 

  2. What seeding density is optimal for the Cloudz Human T Cell Activation Kit, Catalog #  CLD001?

    • A seeding cell density of 0.25 x 106 cells/mL is a good starting density. However, further optimization may be required as there will be donor-to-donor variation.

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