Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CLI-9

Format

BSA Free
View all Clusterin Primary Antibodies »

Product Specifications

Immunogen

Purified human clusterin (TRPM-2).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Clusterin/APOJ Antibody (CLI-9) - BSA Free (NBP1-97521) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Clusterin/APOJ Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Clusterin/APOJ Antibody (CLI-9) - BSA Free

Western Blot: Clusterin/APOJ Antibody (CLI-9)BSA Free [NBP1-97521]

Western Blot: Clusterin/APOJ Antibody (CLI-9)BSA Free [NBP1-97521]

Western Blot: APOJ / Clusterin Antibody (CLI-9) [NBP1-97521] - APOJ / Clusterin Antibody (CLI-9) Detection of natural human Clusterin and FLAG-tagged overexpressed Clusterin in transfected HEK 293T cells. Method: Endogenous human Clusterin and recombinant FLAG-tagged Clusterin from cell lysates were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with a 1:2000 dilution of the CL1-9 antibody on the left or with an anti-FLAG epitope-tag antibody on the right. Proteins were visualized using an HRP-conjugated anti-mouse IgG antibody and a chemiluminescence detection system.

Applications for Clusterin/APOJ Antibody (CLI-9) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Clusterin

APOJ / Clusterin Antibody (CLI-9) (SGP-2/sulfated glycoprotein-2; CLI; Apolipoprotein J (Apo J); TRPM-2) is the first identified extracellular mammalian chaperone and appears to be implicated in such diverse processes as sperm maturation, regulation of complement activation, programmed cell death, tissue remodelling and lipid transport. Increases in clusterin mRNA and protein have been consistently detected in apoptotic cell death paradigms, establishing clusterin gene expression as a prominent marker of apoptotic cell loss. A dual role has been shown for clusterin in that cells can be protected from damage and dead cells can be more easily removed after some types of cellular damage.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Gene Symbol

CLU

Additional Clusterin Products

Product Documents for Clusterin/APOJ Antibody (CLI-9) - BSA Free

Certificate of Analysis

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Product Specific Notices for Clusterin/APOJ Antibody (CLI-9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Clusterin/APOJ Antibody (CLI-9) - BSA Free

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Protocols

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