Clusterin/APOJ Antibody

Novus Biologicals | Catalog # NBP1-06027

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Western Blot, Peptide ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Clusterin Primary Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EKALQEYRRKSRAE corresponding to C-Terminus according to NP_038520.1.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28971894).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Description

Novus Biologicals Goat Clusterin/APOJ Antibody (NBP1-06027) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. Anti-Clusterin/APOJ Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Clusterin/APOJ Antibody

Clusterin/APOJ Antibody

Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody [NBP1-06027] -

Immunocytochemistry/Immunofluorescence: Clusterin/APOJ Antibody [NBP1-06027] - Immunofluorescence analysis of paraformaldehyde fixed PC12 suspension cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Clusterin/APOJ Antibody

Western Blot: Clusterin/APOJ Antibody [NBP1-06027] -

Western Blot: Clusterin/APOJ Antibody [NBP1-06027] - (0.1ug/ml) staining of Mouse (A) and (0.3ug/ml) Rat (B) Brain lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

Applications for Clusterin/APOJ Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Peptide ELISA

Detection limit 1:64000

Western Blot

0.1-0.3 ug/ml
Application Notes
WB: Approx. 38kDa band observed in Mouse and Rat Brain lysates.(calculated MW of 51.7kDa according to Mouse NP_038520.2 and 51.4kDa according to Rat XP_006252156.1). This molecular weight is routinely observed by other sources and thought to correspond to the alpha chain of APOJ. Primary incubation 1 hour at room temperature.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Clusterin

Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP-40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid poly-peptide that is posttranslationally cleaved at an internal bond between Arg 227 and Ser 228. Two subunits, alpha and beta, are associated through disulfide bonds. The beta subunit (also called ApoJalpha) corresponds to residues 23-227. The alpha subunit (also called ApoJbeta) corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences beta-Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer's disease pathogenesis.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Entrez Gene IDs

12759 (Mouse); 24854 (Rat)

Gene Symbol

CLU

UniProt

NP_038520.1 (Mouse)

Additional Clusterin Products

Product Documents for Clusterin/APOJ Antibody

Certificate of Analysis

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Product Specific Notices for Clusterin/APOJ Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Clusterin/APOJ Antibody

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Protocols

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