CNOT8 Antibody (1F11) - Azide and BSA Free
Novus Biologicals | Catalog # H00009337-M01
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 1F11
Format
Azide and BSA Free
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Product Specifications
Immunogen
CNOT8 (NP_004770.4, 201 a.a. ~ 292 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SCKNLKGGLQEVADQLDLQRIGRQHQAGSDSLLTGMAFFRMKELFFEDSIDDAKYCGRLYGLGTGVAQKQNEDVDSAQEKMSILAIINNMQQ
Specificity
Reacts with CCR4-NOT transcription complex, subunit 8.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for CNOT8 Antibody (1F11) - Azide and BSA Free
Immunocytochemistry/ Immunofluorescence: CNOT8 Antibody (1F11) [H00009337-M01]
Immunocytochemistry/Immunofluorescence: CNOT8 Antibody (1F11) [H00009337-M01] - CNOT8 antibody was used (1:50) for labeling and microscopy analysis of the distribution of CNOT8 proteins in MDA-MB-231 cells. Image from verified customer review.
Sandwich ELISA: CNOT8 Antibody (1F11) [H00009337-M01] - Detection limit for recombinant GST tagged CNOT8 is 0.3 ng/ml as a capture antibody.
Applications for CNOT8 Antibody (1F11) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is reactive against recombinant protein in western blot and ELISA. CNOT8 Antibody (1F11) validated for Immunocytochemistry/Immunofluorescence from a verified customer review.
Reviewed Applications
Read 1 review rated 4 using H00009337-M01 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
In 1x PBS, pH 7.4
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: CNOT8
Alternate Names
CAF1, CAF2, CALIFCAF1-like protein, CALIFp, CCR4-NOT transcription complex subunit 8, CCR4-NOT transcription complex, subunit 8, hCAF1, PGK promoter directed over production, POP2CCR4-associated factor 8
Entrez Gene IDs
9337 (Human)
Gene Symbol
CNOT8
UniProt
Additional CNOT8 Products
Product Documents for CNOT8 Antibody (1F11) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CNOT8 Antibody (1F11) - Azide and BSA Free
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Customer Reviews for CNOT8 Antibody (1F11) - Azide and BSA Free (1)
4 out of 5
1 Customer Rating
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Application: ImmunofluorescenceSample Tested: MDA-MB-231 cellSpecies: HumanVerified Customer | Posted 10/20/2022Immunofluorescence (IF): CNOT8 Antibody [H00009337-M01] was used (1:50) for labeling and microscopy analysis of the distribution of CNOT8 proteins in MDA-MB-231 cells.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Immunocytochemistry (ICC) Protocol
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- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
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- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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