CNPase Antibody (8O3B6)

Novus Biologicals | Catalog # NBP3-15622

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8O3B6 expressed in HEK293
View all CNPase Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 322-421 of human CNPase (P09543). NLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNPase Antibody (8O3B6)

CNPase Antibody (8O3B6)

Western Blot: CNPase Antibody (8O3B6) [NBP3-15622] -

Western Blot: CNPase Antibody (8O3B6) [NBP3-15622] - Western blot analysis of various lysates using CNPase Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
CNPase Antibody (8O3B6)

Immunohistochemistry: CNPase Antibody (8O3B6) [NBP3-15622] -

Immunohistochemistry: CNPase Antibody (8O3B6) [NBP3-15622] - Immunohistochemistry analysis of CNPase in paraffin-embedded mouse brain tissue using CNPase Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
CNPase Antibody (8O3B6)

Immunohistochemistry: CNPase Antibody (8O3B6) [NBP3-15622] -

Immunohistochemistry: CNPase Antibody (8O3B6) [NBP3-15622] - Immunohistochemistry analysis of CNPase in paraffin-embedded rat brain tissue using CNPase Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
CNPase Antibody (8O3B6)

Immunohistochemistry: CNPase Antibody (8O3B6) [NBP3-15622] -

Immunohistochemistry: CNPase Antibody (8O3B6) [NBP3-15622] - Immunohistochemistry analysis of CNPase in paraffin-embedded human brain tissue using CNPase Rabbit mAb at a dilution of 1:1600 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
CNPase Antibody (8O3B6)

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (8O3B6) [NBP3-15622] -

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (8O3B6) [NBP3-15622] - Immunofluorescence analysis of paraffin-embedded rat brain using CNPase Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
CNPase Antibody (8O3B6)

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (8O3B6) [NBP3-15622] -

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (8O3B6) [NBP3-15622] - Immunofluorescence analysis of paraffin-embedded mouse brain using CNPase Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for CNPase Antibody (8O3B6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

Additional CNPase Products

Product Documents for CNPase Antibody (8O3B6)

Certificate of Analysis

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Product Specific Notices for CNPase Antibody (8O3B6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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