CNPase Antibody (CL2887) - BSA Free

Novus Biologicals | Catalog # NBP2-46617

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # CL2887

Format

BSA Free
View all CNPase Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P09543, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CNPase Antibody (CL2887) - BSA Free

Western Blot: CNPase Antibody (CL2887) [NBP2-46617]

Western Blot: CNPase Antibody (CL2887) [NBP2-46617]

Western Blot: CNPase Antibody (CL2887) [NBP2-46617] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Mouse Cerebral Cortex tissue
Immunocytochemistry/ Immunofluorescence: CNPase Antibody (CL2887) [NBP2-46617]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (CL2887) [NBP2-46617]

Immunocytochemistry/Immunofluorescence: CNPase Antibody (CL2887) [NBP2-46617] - Staining of RH-30 cells using the Anti-CNP monoclonal antibody, showing specific staining in the cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available). Antibody staining is shown in green.
Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617] - Staining of human pancreas shows absence of immunoreactivity (negative control).
Western Blot: CNPase Antibody (CL2887) [NBP2-46617]

Western Blot: CNPase Antibody (CL2887) [NBP2-46617]

Western Blot: CNPase Antibody (CL2887) [NBP2-46617] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: Human Cerebral Cortex tissue
Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of rat cerebral cortex shows strong immunoreactivity in single oligodendrocytes.
Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of mouse cerebral cortex shows strong immunoreactivity in oligodendrocytes.
Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617] - Staining of human cerebral cortex shows strong immunoreactivity in oligodendrocytes.
Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of rat hippocampal formation shows strong immunoreactivity in oligodendrocytes and myelinated fibers.
Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry: CNPase Antibody (CL2887) [NBP2-46617] - Staining of mouse amygdala shows strong immunoreactivity in oligodendrocytes.
Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617]

Immunohistochemistry-Paraffin: CNPase Antibody (CL2887) [NBP2-46617] - Staining of human cerebellum shows strong immunoreactivity in oligodendrocytes and myelinated neural fibers.

Applications for CNPase Antibody (CL2887) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:5000 - 1:10000

Immunohistochemistry-Paraffin

1:5000 - 1:10000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Entrez Gene IDs

1267 (Human)

Gene Symbol

CNP

UniProt

P09543 (Human)

Additional CNPase Products

Product Documents for CNPase Antibody (CL2887) - BSA Free

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Product Specific Notices for CNPase Antibody (CL2887) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CNPase Antibody (CL2887) - BSA Free

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Protocols

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FAQs

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