CNPase Antibody (CL2887) - BSA Free

Novus Biologicals | Catalog # NBP3-57807

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Mouse IgG2B Clone # CL2887

Format

BSA Free
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Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from P09543, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Analysis in human cerebral cortex and pancreas tissues using NBP3-57807 antibody. Corresponding CNP RNA-seq data are presented for the same tissues.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Analysis in human cerebral cortex tissue.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Mouse Cerebral Cortex tissue
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of human cerebellum shows strong cytoplasmic positivity in oligodendrocytes and in myelinated neural fibers.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of human cerebellum shows strong positivity in oligodendrocytes.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of human cerebral cortex shows strong cytoplasmic positivity in oligodendrocytes.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of human cerebral cortex shows strong positivity in oligodendrocytes.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of human liver shows no positivity in hepatocytes as expected.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of mouse brain shows strong positivity in oligodendrocytes in the amygdala.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of mouse brain shows strong positivity in oligodendrocytes in the cerebral cortex.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of rat brain shows strong positivity in oligodendrocytes in cerebral cortex.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of rat brain shows strong positivity in oligodendrocytes in the hippocampus.
CNPase Antibody (CL2887) - BSA Free

CNPase Antibody (CL2887) - BSA Free

Staining of rat cerebral cortex shows strong immunoreactivity in single oligodendrocytes.

Applications for CNPase Antibody (CL2887) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10000 - 1:20000

Immunohistochemistry-Paraffin

1:10000 - 1:20000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2), 40% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

Additional CNPase Products

Product Documents for CNPase Antibody (CL2887) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for CNPase Antibody (CL2887) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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