CNPase Antibody (JF10-25)

Novus Biologicals | Catalog # NBP2-66986

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # JF10-25 expressed in HEK293
View all CNPase Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide within Human CNPase aa 386-421 / 421. (SwissProt: P09543 Human; SwissProt: P16330 Mouse; SwissProt: P13233 Rat)

Localization

Membrane, Melanosome.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CNPase Antibody (JF10-25)

Western Blot: CNPase Antibody (JF10-25) [NBP2-66986]

Western Blot: CNPase Antibody (JF10-25) [NBP2-66986]

Western Blot: CNPase Antibody (JF10-25) [NBP2-66986] - Western blot analysis of CNPase on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: mouse brain tissue lysate Lane 2: mouse cerebrum tissue lysate
Immunocytochemistry/ Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986]

Immunocytochemistry/Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986] - Staining CNPase in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986]

Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986]

Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of paraffin-embedded rat brain tissue using anti-CNPase antibody. Counter stained with hematoxylin.
Flow Cytometry: CNPase Antibody (JF10-25) [NBP2-66986]

Flow Cytometry: CNPase Antibody (JF10-25) [NBP2-66986]

Flow Cytometry: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of SH-SY-5Y cells with CNPase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986]

Immunocytochemistry/Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986] - Staining CNPase in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/ Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986]

Immunocytochemistry/Immunofluorescence: CNPase Antibody (JF10-25) [NBP2-66986] - Staining CNPase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986]

Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986]

Immunohistochemistry-Paraffin: CNPase Antibody (JF10-25) [NBP2-66986] - Analysis of paraffin-embedded mouse brain tissue using anti-CNPase antibody. Counter stained with hematoxylin.

Applications for CNPase Antibody (JF10-25)

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

1:1000-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

Additional CNPase Products

Product Documents for CNPase Antibody (JF10-25)

Certificate of Analysis

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Product Specific Notices for CNPase Antibody (JF10-25)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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