CNPase Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-50058

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Format

BSA Free
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Product Specifications

Immunogen

Full length human CNPase recombinant protein. [UniProt# P09543]

Clonality

Polyclonal

Host

Rabbit

Theoretical MW

48 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for CNPase Antibody - BSA Free

Western Blot: CNPase Antibody [NBP2-50058]

Western Blot: CNPase Antibody [NBP2-50058]

Western Blot: CNPase Antibody [NBP2-50058] - Analysis of different tissue lysates using rabbit pAb to CNP, NBP2-50058, dilution 1:5,000 in red: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. Double bands at 46, 48kDa mark correspond to isotypes of the CNP protein. The blot was simultaneously probed with mouse mAb to calretinin, dilution 1:2,000, in green. The single band at 29kDa corresponds to the calretinin protein.
Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP2-50058]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP2-50058]

Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP2-50058] - Mixed neuron-glial cell cultures stained with NBP2-50058 (green) and monoclonal antibody against GFAP (red, NBP1-05197). The CNPase antibody stains strongly in oligodendrocytes, whereas GFAP labels the intermediate filament in astrocytes. Blue is DNA staining showing the nuclei of these and numerous other cells, most of which are neurons and which do not express either CNPase or GFAP.

Applications for CNPase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:2000

Immunohistochemistry

1:1000 - 1:2000

Western Blot

1:1000 - 1:5000
Application Notes
In western blot, double bands may be detected at 46 and 48kDa, corresponding to the two isotypes of the CNP protein.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Supplied as serum

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

UniProt

Additional CNPase Products

Product Documents for CNPase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CNPase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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