CNPase Antibody - BSA Free
Novus Biologicals | Catalog # NBP3-05551
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Key Product Details
Species Reactivity
Human, Mouse, Rat, Porcine, Bovine
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Goat
Format
BSA Free
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Product Specifications
Immunogen
Full length human recombinant CNPase expressed in E. coli
Clonality
Polyclonal
Host
Goat
Scientific Data Images for CNPase Antibody - BSA Free
Western Blot: CNPase Antibody [NBP3-05551]
Western Blot: CNPase Antibody [NBP3-05551] - Western blot analysis of different tissue lysates using goat pAb to CNPase at a dilution of 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNPase protein.Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP3-05551]
Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP3-05551] - Immunofluorescent analysis of a section of mouse striatum stained with CNPase Antibody at a dilution of 1:3,000 in green, and costained with rabbit pAb to NF-L at a dilution of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNPase antibody stains myelin sheath and the plasma membranes of oligodendrocytes, the myelin producing cells of the CNS. The NF-L antibody labels axons of neuronal cells enclosed by the myelin.Applications for CNPase Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:3000
Western Blot
1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
50% PBS, 50% glycerol
Format
BSA Free
Preservative
5mM Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.
Background: CNPase
Alternate Names
2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37
Gene Symbol
CNP
Additional CNPase Products
Product Documents for CNPase Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CNPase Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for CNPase Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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