CNPase Antibody - BSA Free

Novus Biologicals | Catalog # NBP3-05551

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat

Format

BSA Free
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Product Specifications

Immunogen

Full length human recombinant CNPase expressed in E. coli

Clonality

Polyclonal

Host

Goat

Scientific Data Images for CNPase Antibody - BSA Free

Western Blot: CNPase Antibody [NBP3-05551]

Western Blot: CNPase Antibody [NBP3-05551]

Western Blot: CNPase Antibody [NBP3-05551] - Western blot analysis of different tissue lysates using goat pAb to CNPase at a dilution of 1:5,000, in green: [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] cow spinal cord, and [7] pig spinal cord. Bands at 46kDa and 48kDa mark correspond to the two major isotypes of CNPase protein.
Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP3-05551]

Immunocytochemistry/ Immunofluorescence: CNPase Antibody [NBP3-05551]

Immunocytochemistry/Immunofluorescence: CNPase Antibody [NBP3-05551] - Immunofluorescent analysis of a section of mouse striatum stained with CNPase Antibody at a dilution of 1:3,000 in green, and costained with rabbit pAb to NF-L at a dilution of 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, the brain was post fixed for 24 hours, cut to 45uM, and free-floating sections were stained with above antibodies. The CNPase antibody stains myelin sheath and the plasma membranes of oligodendrocytes, the myelin producing cells of the CNS. The NF-L antibody labels axons of neuronal cells enclosed by the myelin.

Applications for CNPase Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:3000

Western Blot

1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50% PBS, 50% glycerol

Format

BSA Free

Preservative

5mM Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: CNPase

2,3-cyclic nucleotide-3-phosphodiesterase (CNP) is a membrane bound, microtubule associated protein that is among the most abundant myelin proteins of the CNS. It is thought that CNP may serve as a regulator of tubulin polymerization and of microtubule distribution (Bifulco et al., 2002). It was recently found that CNP may also function as a possible linker protein anchoring microtubules to the plasma membrane via a 13 residue C-terminal CNP fragment (Bifulco et al., 2002, Esposito et al., 2008).

Alternate Names

2', 3' cyclic nucleotide 3' phosphohydrolase, 2'-3'-cyclic nucleotide 3' phosphodiesterase, 2'-3'-cyclic-nucleotide 3'-phosphodiesterase, CNP1, CNPase, EC 3.1.4.37

Gene Symbol

CNP

Additional CNPase Products

Product Documents for CNPase Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for CNPase Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for CNPase Antibody - BSA Free

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Protocols

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