Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44078

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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL3807

Format

Azide and BSA Free
View all Coagulation Factor III/Tissue Factor Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P13726, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images

Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line A-431
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Staining of human kidney shows strong positivity in renal glomerulus.
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Staining of human cerebral cortex shows strong positivity in neuropil.
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Staining of human colon shows strong immunoreactivity in pericryptal sheath cells.
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Staining of human lung cancer shows strong immunoreactivity in a subset of cells.
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Staining of human skeletal muscle shows absence of immunoreactivity (negative control).
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Staining of human placenta shows strong immunoreactivity in blood vessels.
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Western Blot: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-Coagulation Factor III/Tissue Factor antibody. Remaining relative intensity is presented. Loading control: Anti-PPIB.
Coagulation Factor III/Tissue Factor Antibody (CL3807) - Azide and BSA Free Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Immunohistochemistry-Paraffin: Coagulation Factor III/Tissue Factor Antibody [NBP3-44078]

Staining of human testis shows strong positivity in the lamina propria.

Applications

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
IHC-Paraffin HIER pH6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Coagulation Factor III/Tissue Factor

Tissue Factor encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. [provided by RefSeq]

Alternate Names

CD142, F3, Thromboplastin, Tissue Factor

Gene Symbol

F3

Additional Coagulation Factor III/Tissue Factor Products

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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  • Q: Have you tested any of your anti-tissue factor antibodies against pig? I need one for western blot.

    A:

    Unfortunately, none of our Tissue Factor antibodies have yet been tested with pig samples, so we cannot guarantee that any of them will work in pig. Here is a link to Tissue Factor antibodies that have been validated for use in Western blot. If you are interested in testing any of these antibodies against pig samples, you could become eligible for our Innovator's Reward program.

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail