Cofilin 2 Antibody

Novus Biologicals | Catalog # NBP1-33570

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (96%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Cofilin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Cofilin 2. The exact sequence is proprietary.

Reactivity Notes

Zebrafish (89%), Xenopus laevis (87%).

Localization

Nucleus matrix, Cytoplasm, cytoskeleton

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cofilin 2 Antibody

Western Blot: Cofilin 2 Antibody [NBP1-33570]

Western Blot: Cofilin 2 Antibody [NBP1-33570]

Western Blot: Cofilin 2 Antibody [NBP1-33570] - Rat tissue extract (50 ug) was separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody diluted at 1:500.
Immunocytochemistry/ Immunofluorescence: Cofilin 2 Antibody [NBP1-33570]

Immunocytochemistry/ Immunofluorescence: Cofilin 2 Antibody [NBP1-33570]

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP1-33570] - Paraformaldehyde-fixed HeLa, using Cofilin 2 (muscle) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
Western Blot: Cofilin 2 Antibody [NBP1-33570]

Western Blot: Cofilin 2 Antibody [NBP1-33570]

Western Blot: Cofilin 2 Antibody [NBP1-33570] - Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Cofilin 2 Antibody

Western Blot: Cofilin 2 Antibody [NBP1-33570] -

Western Blot: Cofilin 2 Antibody [NBP1-33570] - Various whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Cofilin 2 antibody (NBP1-33570) diluted at 1:1000.

Applications for Cofilin 2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Cofilin 2

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Alternate Names

cofilin 2 (muscle), Cofilin, muscle isoform, cofilin-2, NEM7

Gene Symbol

CFL2

Additional Cofilin 2 Products

Product Documents for Cofilin 2 Antibody

Certificate of Analysis

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Product Specific Notices for Cofilin 2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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