Cofilin 2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92683

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Cofilin 2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-166 of human CFL2 (NP_068733.1). MASGVTVNDEVIKVFNDMKVRKSSTQEEIKKRKKAVLFCLSDDKRQIIVEEAKQILVGDIGDTVEDPYTSFVKLLPLNDCRYALYDATYETKESKKEDLVFIFWAPESAPLKSKMIYASSKDAIKKKFTGIKHEWQVNGLDDIKDRSTLGEKLGGNVVVSLEGKPL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Cofilin 2 Antibody - BSA Free

Western Blot: Cofilin 2 AntibodyBSA Free [NBP2-92683]

Western Blot: Cofilin 2 AntibodyBSA Free [NBP2-92683]

Western Blot: Cofilin 2 Antibody [NBP2-92683] - Western blot analysis of extracts of various cell lines, using Cofilin 2 antibody (NBP2-92683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: Cofilin 2 Antibody - BSA Free [NBP2-92683]

Immunocytochemistry/ Immunofluorescence: Cofilin 2 Antibody - BSA Free [NBP2-92683]

Immunocytochemistry/Immunofluorescence: Cofilin 2 Antibody [NBP2-92683] - Immunofluorescence analysis of A549 cells using Cofilin 2 antibody (NBP2-92683). Blue: DAPI for nuclear staining.

Applications for Cofilin 2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Cofilin 2

Cofilin is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH-dependent manner. (Gillett et al., 1996 [PubMed 8800436]). Cofilin-2 is a member of the AC group of proteins that also includes cofilin-1 (CFL1) and destrin (DSTN; MIM 609114), all of which regulate actin-filament dynamics (Bamburg et al., 1999 [PubMed 10461190]; Maciver and Hussey, 2002). The CFL2 gene encodes a skeletal muscle-specific isoform (Vartiainen et al., 2002 [PubMed 11809832]) localized to the thin filaments, where it exerts its effect on actin, in part through interactions with tropomyosins (Ono and Ono, 2002 [PubMed 11901171]).[supplied by OMIM]

Alternate Names

cofilin 2 (muscle), Cofilin, muscle isoform, cofilin-2, NEM7

Gene Symbol

CFL2

Additional Cofilin 2 Products

Product Documents for Cofilin 2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cofilin 2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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