Cofilin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-1900

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Canine

Cited:

Human, Rat

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western, Microarray

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all CFL1 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acids 154-166 of human cofilin with N-terminal cysteine added, conjugated to maleimide-activated keyhole limpet hemocyanin (KLH). The corresponding immunizing sequence is identical in pig and rat non-muscle cofilin and differs by three amino acids from that of human and chicken muscle cofilin.

Reactivity Notes

Not yet tested in other species.

Localization

Cytoplasmic and Nuclear

Specificity

Detects a band at approximately 19kDa. Reacts with muscle and non-muscle cofilin.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Cofilin Antibody - BSA Free

Western Blot: Cofilin Antibody [NB100-1900]

Western Blot: Cofilin Antibody [NB100-1900]

Western Blot: Cofilin Antibody [NB100-1900] - Whole extract of A-431 Human epithelial carcinoma cells were separated on SDS-PAGE and probed with Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:6000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat.Lanes:1. 1:10,000 antibody2. 1:20,000 antibody3. Negative Control
Immunocytochemistry/ Immunofluorescence: Cofilin Antibody [NB100-1900]

Immunocytochemistry/ Immunofluorescence: Cofilin Antibody [NB100-1900]

Immunocytochemistry/Immunofluorescence: Cofilin Antibody [NB100-1900] - NIH-3T3 Mouse fibroblasts cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.2% Triton X-100. Fixed cells were stained with 1:1,000 Anti-Cofilin antibody produced in Rabbit. The antibody was developed using 1:100 Anti-Rabbit IgG (whole molecule)-FITC antibody produced in Goat. Picture B: cells were counterstained with DAPI (blue) to stain nuclei.

Applications for Cofilin Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000

Western Blot

1:10000
Application Notes

This antibody can be used in Western Blot. For WB use whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, mouse NIH/3T3, and dog MDCK kidney cells. IF only recommended for indirect staining. Staining of cofilin is inhibited by the immunizing peptide. Also suitable for microarray. Not tested in other applications. *Optimal dilutions should be determined by the end user.
See Simple Western Antibody Database for Simple Western validation

Reviewed Applications

Read 1 review rated 1 using NB100-1900 in the following applications:

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: CFL1

Cofilin is a small phosphoinositide sensitive, actin binding protein capable of depolymerizing actin filaments in vitro. Under certain conditions, it fragments the filaments and accelerates actin subunit dissociation from their 'pointed' (minus) ends. Cofilin binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in an apparent pH-dependent, Ca2+ independent manner. Cofilin intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. Cofilin is ubiquitous in tissues of eukaryotes and is especially abundant in neuronal tissues. It is essential for viability and is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, cytokinesis, and pathogen intracellular motility.

Long Name

cofilin 1

Alternate Names

CFL, cofilin, cofilin-1, HEL-S-15

Entrez Gene IDs

1072 (Human); 12631 (Mouse); 29271 (Rat)

Gene Symbol

CFL1

UniProt

P23528 (Human)

Additional CFL1 Products

Product Documents for Cofilin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Cofilin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Cofilin Antibody - BSA Free

Customer Reviews for Cofilin Antibody - BSA Free (1)

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  • Cofilin Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: MDCK canine kidney epithelial cell line
    Species: Canine
    Verified Customer | Posted 07/18/2022
    MDCK cells transfected with GFP-tagged human Cofilin were stained with anti-Cofilin antibody NB100-1900 at 1:1000. Antibody staining showed little correlation with GFP-Cofilin, so the antibody did not appear to be recognizing Cofilin adequately for IF.
    Cofilin Antibody - BSA Free NB100-1900

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