Coilin Antibody (Pdelta) - BSA Free

Novus Biologicals | Catalog # NB600-1275

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse (Negative)

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Microarray

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # Pdelta

Format

BSA Free
View all Coilin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (C-terminus of Human coilin, 389 amino acids).

Epitope

Monoclonal Anti-coilin reacts with an epitope located between amino acids 363 and 481 of human coilin.

Reactivity Notes

Cross-reacts with Human. Does not cross-react with Mouse. Not yet tested in other species.

Localization

Nuclear

Specificity

Coilin (Pdelta)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

~80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Coilin Antibody (Pdelta) - BSA Free

Western Blot: Coilin Antibody (Pdelta)BSA Free [NB600-1275]

Western Blot: Coilin Antibody (Pdelta)BSA Free [NB600-1275]

Western Blot: Coilin Antibody (Pdelta) - BSA Free [NB600-1275] - HeLa cells nuclear extract were separated on SDS-PAGE and probed with 2 ug/mL Monoclonal Anti-Coilin antibody produced in Mouse, Clone: p-delta. The antibody was developed using 1:1000 Anti-Mouse IgG (Fab specific)-Alkaline Phosphatase antibody produced in Goat
Immunocytochemistry/ Immunofluorescence: Coilin Antibody (Pdelta) - BSA Free [NB600-1275]

Immunocytochemistry/ Immunofluorescence: Coilin Antibody (Pdelta) - BSA Free [NB600-1275]

Immunocytochemistry/Immunofluorescence: Coilin Antibody (Pdelta) [NB600-1275] - HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 ug/mL Monoclonal Anti-Coilin Clone: pdelta (NB600-1275). The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Applications for Coilin Antibody (Pdelta) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Western Blot

1-2 ug/ml
Application Notes
WB: Detects a band of approximately 80 kDa. Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Coilin

Coilin is encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.

Alternate Names

CLN80p80-coilin, coilin, coilin p80, p80

Entrez Gene IDs

8161 (Human); 12812 (Mouse)

Gene Symbol

COIL

UniProt

P38432 (Human)

Additional Coilin Products

Product Documents for Coilin Antibody (Pdelta) - BSA Free

Certificate of Analysis

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Product Specific Notices for Coilin Antibody (Pdelta) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Coilin Antibody (Pdelta) - BSA Free

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Protocols

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