COL1A2 Antibody (HL2048) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25414

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2048 expressed in HEK293

Format

Azide and BSA Free
View all COL1A2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment of human COL1A2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COL1A2 Antibody (HL2048) - Azide and BSA Free

COL1A2 Antibody (HL2048) - Azide and BSA Free

Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] -

Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (NBP3-25414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
COL1A2 Antibody (HL2048) - Azide and BSA Free

Immunohistochemistry-Paraffin: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] -

Immunohistochemistry-Paraffin: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] - COL1A2 antibody [HL2048] detects secreted COL1A2 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. COL1A2 stained by COL1A2 antibody [HL2048] (NBP3-25414) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
COL1A2 Antibody (HL2048) - Azide and BSA Free

Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] -

Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (NBP3-25414) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
COL1A2 Antibody (HL2048) - Azide and BSA Free

Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] -

Western Blot: COL1A2 Antibody (HL2048) - Azide and BSA Free [NBP3-25414] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with COL1A2 antibody [HL2048] (NBP3-25414) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for COL1A2 Antibody (HL2048) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:1000-1:10000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COL1A2

The extensive family of COL gene products (collagens) is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue, such as tendons and cartilage. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Several collagens also play a role in cell adhesion, important for maintaining normal tissue architecture and function.

Alternate Names

alpha 2(I)-collagen, Alpha-2 type I collagen, collagen alpha-2(I) chain, collagen I, alpha-2 polypeptide, collagen of skin, tendon and bone, alpha-2 chain, collagen, type I, alpha 2, OI4, osteogenesis imperfecta type IV, type I procollagen

Gene Symbol

COL1A2

Additional COL1A2 Products

Product Documents for COL1A2 Antibody (HL2048) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for COL1A2 Antibody (HL2048) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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