Collagen I alpha 1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-68942

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Validated:

Western Blot, ELISA

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Collagen I alpha 1 Primary Antibodies »

Product Specifications

Immunogen

This Collagen I alpha 1 antibody was raised against synthetic peptides corresponding to COL1A1 (collagen, type I, alpha 1) The peptide sequence was selected from the C terminal of COL1A1. Peptide sequence YRADDANVVRDRDLEVDTTLKSLSQQIENIRSPEGSRKNPARTCRDLKMC. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Rat reactivity reported from a verified customer review.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

139 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for Collagen I alpha 1 Antibody - BSA Free

Western Blot: Collagen I alpha 1 Antibody [NBP1-68942]

Western Blot: Collagen I alpha 1 Antibody [NBP1-68942]

Western Blot: Collagen I alpha 1 Antibody [NBP1-68942] - Titration: 0.2-1 ug/ml, Positive Control: Human brain. Theoretical molecular weight: 139 kDa.
ELISA: Collagen I alpha 1 Antibody [NBP1-68942]

ELISA: Collagen I alpha 1 Antibody [NBP1-68942]

ELISA: Collagen I alpha 1 Antibody [NBP1-68942] - Collagen I levels in rat brain cell lysates. ELISA image submitted by a verified customer review.

Applications for Collagen I alpha 1 Antibody - BSA Free

Application
Recommended Usage

Western Blot

1.0 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP1-68942 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen I alpha 1

Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Collagens are highly conserved and are characterized by an uninterrupted "Glycine X Y" triplet repeat that is a necessary part of the triple helical structure. The extensive family of collagens is composed of several chain types, including fibril-forming interstitial collagens (types I, II, III and V) and basement membrane collagens (type IV), each type containing multiple isoforms. Collagen type I (also known as collagen alpha, COL1A1, and alpha-1 type I collagen) is the largest component of fibrillar collagen found in cartilage and connective tissues. It is synthesized by fibroblasts, osteoblasts, and odontoblasts and has a theoretical molecular weight of 138 kDa.

Type I collagen is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon tissue. Collagens are fibrous, extracellular matrix proteins with high tensile strength and are the major components of connective tissue. Several collagens play a role in cell adhesion, responsible for maintaining normal tissue architecture and function. All collagens contain a triple helix domain and frequently show lateral self-association in order to form complex connective tissues. Post-Golgi LH3 trafficking is essential for collagen homeostasis and for the development and function of multiple organs and tissues (1).

The COL1A1 gene encodes the pro-alpha1 chains of type I collagen protein, whose triple helix is comprised of two alpha1 chains and one alpha2 chain. Mutations in the encoding COL1A1 gene are associated with brittle bone disease (Osteogenesis Imperfecta), cortical hyperostosis (Caffey disease) and disorders that affect the connective tissues (Ehlers-Danlos syndrome) (2). Studies have found that HIF-1 transcription regulation of collagen prolyl hydroxylases regulates collagen deposition, promoting cancer cell alignment along collagen fibers, which enhances invasion and metastasis to lymph nodes and lung tissue by breast cancer cells (3).

References

1. Banushi, B., Forneris, F., Straatman-Iwanowska, A., Strange, A., Lyne, A. M., Rogerson, C.,... Gissen, P. (2016). Regulation of post-Golgi LH3 trafficking is essential for collagen homeostasis. Nat Commun, 7, 12111. doi:10.1038/ncomms12111

2. Lu, Y., Zhang, S., Wang, Y., Ren, X., & Han, J. (2019). Molecular mechanisms and clinical manifestations of rare genetic disorders associated with type I collagen. Intractable Rare Dis Res, 8(2), 98-107. doi:10.5582/irdr.2019.01064

3. Gilkes, D. M., Chaturvedi, P., Bajpai, S., Wong, C. C., Wei, H., Pitcairn, S.,... Semenza, G. L. (2013). Collagen prolyl hydroxylases are essential for breast cancer metastasis. Cancer Res, 73(11), 3285-3296. doi:10.1158/0008-5472.Can-12-3963

Alternate Names

COL1A1, OI4

Gene Symbol

COL1A1

UniProt

P02452

Additional Collagen I alpha 1 Products

Product Documents for Collagen I alpha 1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Collagen I alpha 1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen I alpha 1 Antibody - BSA Free

Customer Reviews for Collagen I alpha 1 Antibody - BSA Free (1)

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  • Collagen I alpha 1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Rat Brain lysates
    Species: Rat
    Verified Customer | Posted 04/26/2021
    Collagen I levels in rat brain lysates.
    Collagen I alpha 1 Antibody - BSA Free NBP1-68942

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FAQs for Collagen I alpha 1 Antibody - BSA Free

Showing  1 - 5 of 5 FAQs Showing All

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

  • Q: How long does delivery take for your collagen 1 alpha 1 antibody?

    A: Our most popular collagen I alpha 1 antibody (NB600-408) is usually in stock and ships priority overnight for next business day delivery in the U.S.

  • Q: I am looking for anti collagen 1 antibody, I see your product calls it collagen I alpha 1 is there a difference?

    A: Collagen I alpha 1 is the most common protein for the collagen 1 protein. There are other subunits as well (such as collagen 1 alpha 2…etc.).

  • Q: The predicted molecular weight says 150kDA but the band on the gel appears at 130kDA why is that?

    A: Differences between the predicted and observed MW can be affected by a number of factors including PTMs, relative charges and experimental factors. These cumulative effect of these differences can be quite substantial with larger molecules such as collagen I alpha 1.

  • Q: We are looking for an anti-Collagen antibody for Flow Cytometry (FACS) analysis in Swine sample. Would you please provide some references if there is any available as well?

    A:

    We currently have one antibody for porcine Collagen validated in flow cytometry (catalog # NBP1-94202).

  • Q: What secondary antibody do you recommend for collagen I alpha 1 antibodies?

    A: Because this collagen I alpha 1 antibody is raised in rabbit you would want to use any anti-rabbit IgG secondary antibody. NB7160 is a popular choice for use in WB.

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