Collagen III alpha 1/COL3A1 Native Protein

Novus Biologicals | Catalog # NBP1-97267

Novus Biologicals
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Key Product Details

Accession Number

P02461

Applications

Immunohistochemistry, Western Blot, ELISA, Immunoprecipitation, SDS-PAGE
View all Collagen III alpha 1/COL3A1 Proteins and Enzymes »

Product Specifications

Description

Native Human Collagen III alpha 1/COL3A1

Source: Human placenta (Accession #:P02461)

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Purity

Chromatography

Applications

Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)
ELISA (1:10000)
Immunoprecipitation (1:100-1:500)

Application Notes

This product has been tested in SDS-Page and western blot and is used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation, and for other immunological assays as optimized by the user. Specific conditions should be optimized by user. Collagen Type III is recognized by type specific anti-collagen antibodies that recognize a native three-dimensional structure.

Protein / Peptide Type

Native Protein

Scientific Data Images for Collagen III alpha 1/COL3A1 Native Protein

Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267]

Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267]

Western Blot: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - Western Blot of Collagen III alpha 1/COL3A1. Lane 1: Human Collagen III. Load: 100 ng per lane. Primary antibody: Collagen III Antibody at 1:1000 at 4C. Secondary antibody: DyLight(TM) 649 Goat anti-rabbit at 1:20,000 for 30 min at RT. Block for 30 min at RT. Predicted/Observed size: 138 kDa, 138 kDa.
SDS-PAGE: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267]

SDS-PAGE: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267]

SDS-Page: Collagen III alpha 1/COL3A1 Native Protein [NBP1-97267] - SDS-PAGE Results of Collagen III alpha 1/COL3A1 Native protein. Lane 1: Collagen III alpha 1/COL3A1 Native protein Reduced [1.0ug]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Collagen III alpha 1/COL3A1 Native protein Non-Reduced [1.0ug]. 4-20% Gel Coomassie Stained. Observed: alpha1 bands.

Formulation, Preparation, and Storage

NBP1-97267
Preparation Method Prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.
Formulation 0.5 M Acetic Acid
Preservative 0.01% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C. Do not freeze.

Background: Collagen III alpha 1/COL3A1

Collagen III alpha 1, also referred to as collagen type III alpha 1 or COL3A1 for short, was first described in 1971 and is a member of the collagen superfamily and encoded COL3A1 gene (1, 2). In general, collagen III is an extracellular matrix protein that is synthesized as a preprocollagen followed by cleaving of the signal peptide to form the procollagen (1). The human COL3A1 gene is located on chromosome 2q32.2 and collagen III is synthesized as a homotrimer consisting of three identical alpha procollagen chains which are stabilized by disulfide bonds (1,2,3). Each alpha chain is 1466 amino acids (aa) in length with a theoretical molecular weight of 139 kDa for a single alpha chain (1). Structurally, each alpha chain is a left-handed helix which then join together to form a right-handed triple helix (1,2). C-terminal and N-terminal proteinases remove the globular ends of the procollagen to form the type III collagen (1).

Collagen III is a fibrillar collagen that constitutes 5-20% of all collagen in the body (1). It provides structural integrity and is found in many hallow organs and soft connective tissue including the vascular system, skin, lung, uterus, and intestine (1,2). Additionally, collagen III has be found to be associated with type I collagen in the same fibrils (1). Collagen III interacts with signaling integrins to carry out other key functions including cell adhesion, proliferation, and differentiation (1).

Mutations in the COL3A1 gene has been associated with a variety of human diseases, the most well-known being a group of connective tissue disorders termed Ehlers-Danlos Syndromes (1,2,4). Vascular Ehlers-Danlos Syndrome is a specific subtype that is considered the most severe and although the clinical manifestations vary, symptoms include thin skin and fragile blood vessels and can often result in both lung and heart complications (1,4). COL3A1 is also associated with glomerulopathies, or diseases of the glomeruli, which are characterized by an abundance of extracellular matrix (3). Collagenofibrotic glomerulopathy is one specific rare renal disease that is characterized by excessive levels of collagen III (3).

References

1. Kuivaniemi, H., & Tromp, G. (2019). Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. Gene. https://doi.org/10.1016/j.gene.2019.05.003

2. Ricard-Blum S. (2011). The collagen family. Cold Spring Harbor perspectives in biology. https://doi.org/10.1101/cshperspect.a004978

3. Cohen A. H. (2012). Collagen Type III Glomerulopathies. Advances in chronic kidney disease. https://doi.org/10.1053/j.ackd.2012.02.017

4. Olson, S. L., Murray, M. L., & Skeik, N. (2019). A Novel Frameshift COL3A1 Variant in Vascular Ehlers-Danlos Syndrome. Annals of vascular surgery. https://doi.org/10.1016/j.avsg.2019.05.057

Alternate Names

COL3A1, EDS4A

Gene Symbol

COL3A1

UniProt

P02461 (Human)

Additional Collagen III alpha 1/COL3A1 Products

Product Documents for Collagen III alpha 1/COL3A1 Native Protein

Certificate of Analysis

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Product Specific Notices for Collagen III alpha 1/COL3A1 Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Citations for Collagen III alpha 1/COL3A1 Native Protein

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Protocols

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FAQs for Collagen III alpha 1/COL3A1 Native Protein

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  • Q: I'm interested in using colagen III in adhesion assays of cell lines to collagen-coated TC plates. I've seen you have human recombinant collagen III (H00001281-P01) but in the datasheet it says it is not suitable for functional assays. I was wondering, though, if it is OK to use it in adhesion assays. You also have non-recombinant collagen III (NBP1-97267), which I guess it's purified. Is this one suitable for adhesion assays?

    A: H00001281-P01 product is distributed by us through a Taiwanese company called Abnova. They do not test the activity of their recombinant proteins. The proteins are produced in a cell-free wheat germ system with an N-terminal 26kDa GST-tag. Due to this expression system, in our experience, the proteins do not always undergo the same folding and post-transnational modifications they might undergo in an endogenous cell. Because of this, we do not recommend this protein for use in functional assays and do not guarantee them for this use. They are mainly intended for use in Western Blots as a positive control with their antibody. NBP1-97267 is a native protein purified from a human source.

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