Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-25416
Recombinant Monoclonal Antibody
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Rat (90%). Backed by our 100% Guarantee.
Applications
Multiplex Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, COMET
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # HL1351 expressed in HEK293
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the N-terminus of human Collagen IV alpha 1. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Bovine (82%).
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
161 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free
Detection of Collagen IV in Human Liver via Multiplex Immunofluorescence staining on COMET™
Collagen IV was detected in immersion fixed paraffin-embedded sections of human liver using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha 1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
Detection of Collagen IV in Human Tonsil via Multiplex Immunofluorescence staining on COMET™
Collagen IV was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha 1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
Detection of Collagen IV in Human Kidney via Multiplex Immunofluorescence staining on COMET™
Collagen IV was detected in immersion fixed paraffin-embedded sections of human Kidney using Rabbit Anti-Human/Mouse/Rat Collagen IV alpha 1 Monoclonal Antibody (Novus Catalog # NBP3-25416) at 1µg/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 555 Goat anti-Rabbit IgG Secondary Antibody at 1:100 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR555RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cell membrane. Protocol available in COMET™ Panel Builder.
Immunocytochemistry/ Immunofluorescence: Collagen IV alpha 1 Antibody (HL1351) [NBP3-25416] -
COL4A1 antibody [HL1351] detects COL4A1 protein at vesicle by immunofluorescent analysis.Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Blue: Fluoroshield with DAPI.
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody (HL1351) [NBP3-25416] -
COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody (HL1351) [NBP3-25416] -
COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane and secreted by immunohistochemical analysis.Sample: Paraffin-embedded mouse placenta.
COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Western Blot: Collagen IV alpha 1 Antibody (HL1351) [NBP3-25416] -
Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Immunohistochemistry-Paraffin: Collagen IV alpha 1 Antibody (HL1351) [NBP3-25416] -
COL4A1 antibody [HL1351] detects COL4A1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.
COL4A1 stained by COL4A1 antibody [HL1351] (NBP3-25416) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Applications for Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
Assay dependent
Immunohistochemistry
Assay dependent
Immunohistochemistry-Paraffin
Assay dependent
Multiplex Immunofluorescence
1ug/mL
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Collagen IV alpha 1
Alternate Names
Arresten, COL4A1
Gene Symbol
COL4A1
Additional Collagen IV alpha 1 Products
Product Documents for Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Collagen IV alpha 1 Antibody (HL1351) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
