Collagen IV Native Protein

Novus Biologicals | Catalog # NBP1-97268

Novus Biologicals
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Key Product Details

Accession Number

P02462

Applications

Immunohistochemistry, Western Blot, ELISA, SDS-PAGE
View all Collagen IV Proteins and Enzymes »

Product Specifications

Description

Native Human Collagen IV

Source: Human placenta (Accession #:CAA00084.1)

Store vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use.

No test method can provide total assurance that the hepatitis B virus, hepatitis C virus, human immunodeficiency virus, or any other infectious agents are absent. Thus, all blood products, including purified proteins derived from human blood sources, should be handled at Biosafety Level 2 as recommended by the CDC\NIH manual entitled Biosafety in Microbiological and Biomedical Laboratories for potentially infectious human serum, blood specimens or proteins derived from same. Source material for the human blood product supplied to your facility has been tested for the detection of HIV antibody, Hepatitis B surface antigen, antibody to Hepatitis C, HIV 1 antigen(s), antibody to HTLV - I/II, and syphilis by FDA guidelines. All units were found to be non-reactive/negative for these tests. All human blood source material is collected in FDA licensed centers and is tested with FDA approved test kits.

Purity

Chromatography

Applications

Immunohistochemistry (1:100-1:500)
Western Blot (1:1000)
ELISA (1:10000)

Application Notes

This product has been tested in SDS-Page and can be used as a control or standard in indirect trapping ELISA for quantitation of antigen using a standard curve, for immunoprecipitation and for other immunological assays as optimized by the user. Specific conditions should be optimized by user.

Protein / Peptide Type

Native Protein

Scientific Data Images for Collagen IV Native Protein

SDS-PAGE: Collagen IV Native Protein [NBP1-97268]

SDS-PAGE: Collagen IV Native Protein [NBP1-97268]

SDS-Page: Collagen IV Native Protein [NBP1-97268] - Human Collagen Type IV. Lane 1: Human Collagen Type IV Reduced (5 ug). Lane 2: Prestained Molecular Weight Protein. Lane 3: Human Collagen Type IV Non-Reduced (5 ug). 4-20% Gel, Coomassie Stained. Observed MW: Type IV a1, a2, a3 bands.

Formulation, Preparation, and Storage

NBP1-97268
Preparation Method Prepared from human placenta and is chromatographically and immunologically pure. This product is free from other collagens, human serum proteins and non-collagen extracellular matrix proteins.
Formulation 0.5 M Acetic Acid
Preservative 0.01% Sodium Azide
Concentration Please see the vial label for concentration. If unlisted please contact technical services.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C. Do not freeze.

Background: Collagen IV

Collagen type IV is a type of collagen found primarily in the basal lamina. The type IV collagen C4 domain at the C-terminus is not removed in post-translational processing, and the fibers link head-to-head, rather than in parallel. Also, collagen type IV lacks the regular glycine in every third residue necessary for the tight, collagen helix. This makes the overall arrangement more sloppy with kinks. These two features cause collagen IV to form in a sheet, the form of the basal lamina. There are six human genes associated with collagen IV: COL4A1, COL4A2, COL4A3, COL4A4, COL4A5 and COL4A6.

Alternate Names

BSVD;BSVD1;COL4A1s;Collagen alpha-1(IV) chain;PADMAL;RATOR

Gene Symbol

COL4A1

UniProt

P02462 (Human)

Additional Collagen IV Products

Product Documents for Collagen IV Native Protein

Certificate of Analysis

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Product Specific Notices for Collagen IV Native Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

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Protocols

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FAQs for Collagen IV Native Protein

Showing  1 - 2 of 2 FAQs Showing All

  • Q:

    Here a customer intends to buy our Collagen IV Protein. He wonders whether it can be used for cell culture, if it can, what concentration is best. Can you so kind to help him and me?

    A: Our Collagen IV protein with catalogue number NBP1-97268 has been validated for Western blotting and for ELISA. We have no testing data regarding its use in cell culture, however since it contains sodium azide as a preservative it is unlikely to be suitable for this use.

  • Q: Which was the best human Collagen IV recombinant protein for my custom sandwich Elisa?

    A: NBP1-97268 is currently our only human Collagen IV protein. It is the native protein and is not recombinant. Without knowing which antibodies are being used in your ELISA, I can not be 100% certain that this is the best protein for you but it is the only one that we carry of human origin.

  • Q:

    Here a customer intends to buy our Collagen IV Protein. He wonders whether it can be used for cell culture, if it can, what concentration is best. Can you so kind to help him and me?

    A: Our Collagen IV protein with catalogue number NBP1-97268 has been validated for Western blotting and for ELISA. We have no testing data regarding its use in cell culture, however since it contains sodium azide as a preservative it is unlikely to be suitable for this use.

  • Q: Which was the best human Collagen IV recombinant protein for my custom sandwich Elisa?

    A: NBP1-97268 is currently our only human Collagen IV protein. It is the native protein and is not recombinant. Without knowing which antibodies are being used in your ELISA, I can not be 100% certain that this is the best protein for you but it is the only one that we carry of human origin.

Showing  1 - 2 of 2 FAQs Showing All
View all FAQs for Proteins and Enzymes