Collagen V Antibody (1E2-E4/Col5) - BSA Free

Novus Biologicals | Catalog # NBP1-05118

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Porcine, Bovine, Canine, Chicken (Negative), Guinea Pig (Negative), Kangaroo, Mouse (Negative), Rabbit, Rat (Negative), Sheep

Cited:

Porcine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1E2-E4/Col5

Format

BSA Free
View all Collagen V Primary Antibodies »

Product Specifications

Immunogen

Acid-digested pepsin soluble dog type V collagen

Specificity

Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and immunoblotting. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for Collagen V Antibody (1E2-E4/Col5) - BSA Free

Western Blot: Collagen V Antibody (1E2-E4/Col5)BSA Free [NBP1-05118]

Western Blot: Collagen V Antibody (1E2-E4/Col5)BSA Free [NBP1-05118]

Western Blot: Collagen V Antibody (1E2-E4/Col5) [NBP1-05118] - Staining with 1E2-E4/Col5 on Dog Type I, III, V and Human Type I, III, and V in rows 1-6 respectively. (1. Werkmeister)

Applications for Collagen V Antibody (1E2-E4/Col5) - BSA Free

Application
Recommended Usage

ELISA

1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05118 can be used for detection of collagens by ELISA. NBP1-05118 binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well. WB: In immunoblotting NBP1-05118 detects human type V collagen only in its native triple helix form. IHC: NBP1-05118 has been used succesfully for immunohistology on paraffin embedded (1) and frozen unfixed sections of human (2), bovine (3) and dog (4) skin, on rabbit (1) and foetal bovine cornea (4), and of new dog tissue associated with a biomaterial implant (5). If fixation of tissue is required, acetone or ethanol is recommended.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Collagen V

Type V collagen is a minor component of the connective tissue, although it is present in many different types of connective tissue. Patients with defects in the type V collagen (Ehlers-Danlos syndrome) have weakened connective tissue characterized by hyperstrechable joints and fragile, easily bruisable skin.

Alternate Names

alpha 1 type V collagen, Collagen 5, collagen alpha-1(V) chain, collagen, type V, alpha 1, Collagen-5, EC 2.7.7.6, EC 6.1.1

Gene Symbol

COL5A1

Additional Collagen V Products

Product Documents for Collagen V Antibody (1E2-E4/Col5) - BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen V Antibody (1E2-E4/Col5) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen V Antibody (1E2-E4/Col5) - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Collagen V Antibody (1E2-E4/Col5) - BSA Free

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  • Q: I can't find the recommended dilutions of NBP1-05118 to do Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin and Western Blot in your datasheet. Please tell me, thank you.

    A: We recommend starting dilutions of: WB, 1:1000-1:2000 and IHC 1:100-1:200. The antibody should be less diluted in IHC-P than IHC-F as the epitope is harder to find in IHC-P. These are recommended dilutions and you should optimize these in order to get the best result.

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