Collagen VI alpha 1 Antibody (SD83-03)

Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney

Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunocytochemistry/Immunofluorescence: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human liver tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human colon tissue with Rabbit anti-Collagen VI alpha 1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Analysis of paraffin-embedded human skin tissue with Rabbit anti-Collagen VI antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Immunohistochemistry-Paraffin: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825] - Immunofluorescence analysis of paraffin-embedded human liver tissue labeling Collagen VI with Rabbit anti-Collagen VI antibody washed with PBS, and then probed with the primary antibody (green) at 1/100 dilution overnight at 4, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor(TM) 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Western Blot: Collagen VI alpha 1 Antibody (SD83-03) [NBP2-67825]

Western blot analysis on different lysates with NBP2-67825 at 1/1,000 dilution. Lane 1: Saos-2 cell lysate Lane 2: NIH/3T3 cell lysate Lane 3: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 145 kDa Observed band size: 145 kDa Exposure time: 6 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67825 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.