Collagen VII Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-37900

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Collagen VII Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LTLYTLLEGHEVATPATVVPTGPELPVSPVTDLQATELPGQRVRVSWSPVPGATQYRIIVRSTQGVERTLVLPGSQTAFDLDDVQAGLSYTVRVSARVGPREGSASVLTVRREPETPLAVPGLRVVVSDATRVRVAWGPAPGASGFRI

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (81%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Collagen VII Antibody - BSA Free

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Analysis in human skin and liver tissues using antibody. Corresponding COL7A1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skin shows moderate membranous positivity in epidermal cells.
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900]

Immunohistochemistry-Paraffin: Collagen VII Antibody [NBP2-37900] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.

Applications for Collagen VII Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Collagen VII

Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.

Alternate Names

Collagen 7, collagen alpha-1(VII) chain, collagen VII, alpha-1 polypeptide, collagen, type VII, alpha 1, Collagen-7, EBD1, EBDCT, EBR1, epidermolysis bullosa, dystrophic, dominant and recessive, LC collagen, long chain collagen, Long-chain collagen

Gene Symbol

COL7A1

UniProt

Q02388 (Human)

Additional Collagen VII Products

Product Documents for Collagen VII Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Collagen VII Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Collagen VII Antibody - BSA Free

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Protocols

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