CometAssay Electrophoresis System II
CometAssay Electrophoresis System II SummaryEnables optimization and standardization of alkaline and neutral comet assays.
• Maintains constant buffer temperature with cooling pack chamber and one-piece molded plastic body.
• Maintains optimal buffer level for consistent results with overlay.
• Specially designed trays accommodate 2, 20 and 96 well slides and maintain correct position during electrophoresis.
• Optimized for use with CometAssay Kits and CometAssay Control Cells.
What is the CometAssay?
The Comet assay, or single cell gel electrophoresis assay, provides a simple and effective method for evaluating DNA damage in cells. The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a current is applied. Evaluation of the DNA “comet” tail shape and migration pattern allows for assessment of DNA damage. The general Comet assay protocol includes first immobilizing cells in a bed of low melting point agarose, gently lysing the cells, treating cells with neutral or alkali solutions to unwind and denature the DNA as well as hydrolyze sites of damage. DNA is then subjected to electrophoresis and stained with a fluorescent DNA intercalating dye.
Why Use the CometAssay Electrophoresis System?
CometAssay ES Il enables investigators to consistently optimize alkaline and neutral comet assays for highly reproducible results, and to standardize electrophoresis methods between individual users and laboratories. Comet assay results can be variable depending on temperature, distance between electrodes, and buffer height. This specialized electrophoresis unit helps resolve these variables.
For research use only. Not for diagnostic use.
Citations for CometAssay Electrophoresis System II
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 10
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Genetic instability from a single S phase after whole-genome duplication
Authors: S Gemble, R Wardenaar, K Keuper, N Srivastava, M Nano, AS Macé, AE Tijhuis, SV Bernhard, DCJ Spierings, A Simon, O Goundiam, H Hochegger, M Piel, F Foijer, Z Storchová, R Basto
Nature, 2022;604(7904):146-151. 2022
CHK1 phosphorylates PRIMPOL to promote replication stress tolerance
Authors: KPM Mehta, V Thada, R Zhao, A Krishnamoo, M Leser, K Lindsey Ro, D Cortez
Science Advances, 2022;8(13):eabm0314. 2022
Topoisomerase 2&beta Induces DNA Breaks To Regulate Human Papillomavirus Replication
Authors: P Kaminski, S Hong, T Kono, P Hoover, L Laimins
MBio, 2021;12(1):. 2021
Aldehyde dehydrogenase inhibitors promote DNA damage in ovarian cancer and synergize with ATM/ATR inhibitors
Authors: E Grimley, AJ Cole, TT Luong, SC McGonigal, S Sinno, D Yang, KA Bernstein, RJ Buckanovic
Theranostics, 2021;11(8):3540-3551. 2021
The long-noncoding RNA SOCS2-AS1 suppresses endometrial cancer progression by regulating AURKA degradation
Authors: F Jian, X Che, J Zhang, C Liu, G Liu, Y Tang, W Feng
Cell Death & Disease, 2021;12(4):351. 2021
SLFN11 is Widely Expressed in Pediatric Sarcoma and Induces Variable Sensitization to Replicative Stress Caused By DNA-Damaging Agents
Authors: J Gartrell, M Mellado-La, MR Clay, A Bahrami, NA Sahr, A Sykes, K Blankenshi, L Hoffmann, J Xie, HP Cho, N Twarog, M Connelly, KK Yan, J Yu, SN Porter, SM Pruett-Mil, G Neale, CL Tinkle, SM Federico, EA Stewart, AA Shelat
Molecular Cancer Therapeutics, 2021;20(11):2151-2165. 2021
Photomutagenicity of chlorpromazine and its N-demethylated metabolites assessed by NGS
Authors: JAG Agúndez, E García-Mar, G García-Lai, MA Miranda, I Andreu
Sci Rep, 2020;10(1):6879. 2020
Hypoosmotic stress induces R loop formation in nucleoli and ATR/ATM-dependent silencing of nucleolar transcription
Authors: AK Velichko, NV Petrova, AV Luzhin, OS Strelkova, N Ovsyanniko, II Kireev, NV Petrova, SV Razin, OL Kantidze
Nucleic Acids Res., 2019;0(0):. 2019
TRIM66 reads unmodified H3R2K4 and H3K56ac to respond to DNA damage in embryonic stem cells
Authors: J Chen, Z Wang, X Guo, F Li, Q Wei, X Chen, D Gong, Y Xu, W Chen, Y Liu, J Kang, Y Shi
Nat Commun, 2019;10(1):4273. 2019
The human Shu complex functions with PDS5B and SPIDR to promote homologous recombination
Authors: J Martino, GJ Brunette, J Barroso-Go, TN Moiseeva, CM Smith, CJ Bakkenist, RJ O'Sullivan, KA Bernstein
Nucleic Acids Res., 2019;47(19):10151-10165. 2019
FBH1 promotes DNA double-strand breakage and apoptosis in response to DNA replication stress.
Authors: Jeong Y, Rossi M, Cermak L, Saraf A, Florens L, Washburn M, Sung P, Schildkraut C, Pagano M
J Cell Biol, 0;200(2):141-9. 0
Androgen receptor signaling regulates DNA repair in prostate cancers.
Authors: Polkinghorn W, Parker J, Lee M, Kass E, Spratt D, Iaquinta P, Arora V, Yen W, Cai L, Zheng D, Carver B, Chen Y, Watson P, Shah N, Fujisawa S, Goglia A, Gopalan A, Hieronymus H, Wongvipat J, Scardino P, Zelefsky M, Jasin M, Chaudhuri J, Powell S, Sawyers C
Cancer Discov, 0;3(11):1245-53. 0
Will any commercial horizontal gel electrophoresis device work for the Comet Assay?
Electrophoresis conditions described for FLARE™ Assay, CometAssay® and CometChip® Kits were optimized for the CometAssay® Electrophoresis System (Catalog # 4250-050-ES). Conventional slab gel electrophoresis chambers are not designed to eliminate known causes of comet assay variability (alkaline pH, buffer height, temperature, slide orientation). They can be used, but they require optimization by the user to achieve consistent results.
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