COMP/Thrombospondin-5 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-92733

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COMP/Thrombospondin-5 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2). GQGQSPLGSDLGPQMLRELQETNAALQDVRELLRQQVREITFLKNTVMECDACGMQQSVRTGLPSVRPLLHCAPGFCFPGVACIQTESGARCGPCPAGFTGNGSHCTDVNECNAHPCFPRVRCINTSPGFRCEACPPGYSG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for COMP/Thrombospondin-5 Antibody - BSA Free

Western Blot: COMP/Thrombospondin-5 AntibodyBSA Free [NBP2-92733]

Western Blot: COMP/Thrombospondin-5 AntibodyBSA Free [NBP2-92733]

Western Blot: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of extracts of various cell lines, using COMP/Thrombospondin-5 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: COMP/Thrombospondin-5 Antibody - BSA Free [NBP2-92733]

Immunocytochemistry/ Immunofluorescence: COMP/Thrombospondin-5 Antibody - BSA Free [NBP2-92733]

Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of L929 cells using COMP Rabbit pAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
COMP/Thrombospondin-5 Antibody

Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] -

Immunocytochemistry/Immunofluorescence: COMP/Thrombospondin-5 Antibody [NBP2-92733] - Analysis of Human Dermal Fibroblasts (HDFa). Green: 1:100 COMP/Thrombospondin-5 antibody in PBS/BSA 1%, 1h room temperature Blue: DAPI. Image from verified customer review.

Applications for COMP/Thrombospondin-5 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:500 - 1:2000

Reviewed Applications

Read 1 review rated 4 using NBP2-92733 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COMP/Thrombospondin-5

COMP is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF like and calcium binding (thrombospondin like) domains. Oligomerization results from formation of a five stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED)

Long Name

Cartilage Oligomeric Matrix Protein

Alternate Names

EDM1, EPD1, MED, PSACH, Thrombospondin-5, Thrombospondin5

Gene Symbol

COMP

Additional COMP/Thrombospondin-5 Products

Product Documents for COMP/Thrombospondin-5 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COMP/Thrombospondin-5 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Extracellular Matrix Molecules

Customer Reviews for COMP/Thrombospondin-5 Antibody - BSA Free (1)

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Customer Images

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  • COMP/Thrombospondin-5 Antibody
    Name: Patricia Carulla Martí
    Application: Immunocytochemistry
    Sample Tested: fibroblasts
    Species: Human
    Verified Customer | Posted 05/25/2023
    Human Dermal Fibroblasts (HDFa) Green: 1:100 COMP/Thrombospondin-5 antibody in PBS/BSA 1%, 1h room temperature Blue: DAPI
    COMP/Thrombospondin-5 Antibody - BSA Free NBP2-92733

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Protocols

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FAQs for COMP/Thrombospondin-5 Antibody - BSA Free

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  • Q: I have synovial fluid sample and I want to work on Western bloting can you support me,how can I prepare this sample for WB technique for assay comp(cartilage oligomatrix protein )?

    A:

    We currently sell four antibodies to cartilage oligomeric matrix protein (COMP), all of which are validated for Western blotting. Although we have generated Western blot data using samples from human, bovine and rabbit cartilage, we do not have any testing data derived from synovial fluid samples. You may however find the following publication useful, in which the authors sampled synovial fluid from patients with rheumatoid arthritis and prepared this fluid for Western blot detection of the proteins SP-A and SP-D: PMC 2080374

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Associated Pathways

Articular Cartilage Extracellular Matrix Articular Cartilage Extracellular Matrix Thumbnail