Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free

Novus Biologicals | Catalog # NB100-65510

Novus Biologicals

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 053A-1149.3.1.4 (1003)

Format

BSA Free
View all Complement C3d Primary Antibodies »

Product Specifications

Immunogen

Purified C3d from human plasma

Specificity

NB100-65510 is specific for human complement component 3d (C3d), a 33kDa polypeptide fragment generated over the course of complement activation where complement 3 (C3) convertases cleave C3 to C3b, which is further degraded into iC3b and C3dg/C3d. C3d is involved in the regulation of many aspects of the immune response, including antigen processing and presentation. The C3d/antigen complex mediates B cell activation by simultaneously binding antigen-specific surface bound immunoglobulin and CD21, lowering the complement activation threshold. C3 is crucial in the induction of tolerance generated when an antigen is introduced into immunoprivileged sites and this is exploited by pathogens and cancer cells to evade the immune system by inhibiting complement activation.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free

Immunohistochemistry-Paraffin: Complement C3d Antibody (053A-1149.3.1.4) - BSA Free [NB100-65510]

Immunohistochemistry-Paraffin: Complement C3d Antibody (053A-1149.3.1.4) - BSA Free [NB100-65510]

Immunohistochemistry-Paraffin: Complement C3d Antibody (053A-1149.3.1.4) [NB100-65510] - Immunohistochemistry staining for C3d in human renal biopsy. IHC-P image submitted by a verified customer review.

Applications for Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 1 review rated 5 using NB100-65510 in the following applications:

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Borate buffered saline (pH 8.4)

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement C3d

The complement factor C3 consists of an alpha and a beta chain. C3 is a central factor in the complement cascade. It is central to the alternative pathway that leads to the C3 convertase C3bBb. The classical mannose binding lectin activation pathway leads to the C3 convertase C4b2a. These convertases cleave C3 resulting in C3a and C3b. Further degradation leads to the formation of the alpha chain products C3d, C3g and C3c. C3 is an acute phase protein that is produced by a wide range of tissues, including renal epithelial cells and hepatocytes.

Alternate Names

AHUS5, ARMD9, ASP, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1, complement C3, complement component 3, complement component C3, CPAMD1acylation-stimulating protein cleavage product

Entrez Gene IDs

718 (Human)

Gene Symbol

C3

UniProt

P01024

Additional Complement C3d Products

Product Documents for Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free

Customer Reviews for Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free (1)

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Customer Images

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  • Complement C3d Antibody (053A-1149.3.1.4)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Renal biopsy specimens and Renal biopsy specimen
    Species: Human
    Verified Customer | Posted 08/05/2021
    Immunohistochemistry staining for C3d in renal biopsy
    Complement C3d Antibody (053A-1149.3.1.4 (1003)) - BSA Free NB100-65510

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