Complement Component C1qA Antibody (7H8)
Novus Biologicals | Catalog # NB200-539
![Immunocytochemistry/ Immunofluorescence: Complement Component C1qA Antibody (7H8) [NB200-539]](https://resources.rndsystems.com/images/products/Complement-Component-C1qA-Antibody-7H8-Chromatin-Immunoprecipitation-NB200-539-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Complement Component C1qA Antibody (7H8) [NB200-539]")
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Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Frozen, Immunoassay, Immunocytochemistry/ Immunofluorescence
Cited:
Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 7H8
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Product Specifications
Immunogen
Unfortunately, this information is considered to be commercially sensitive
Localization
Cytoplasmic and Secreted
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Description
Novus Biologicals Rat Complement Component C1qA Antibody (7H8) (NB200-539) is a monoclonal antibody validated for use in IHC and ICC/IF. Anti-Complement Component C1qA Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Complement Component C1qA Antibody (7H8)
Immunocytochemistry/ Immunofluorescence: Complement Component C1qA Antibody (7H8) [NB200-539]
Immunocytochemistry/Immunofluorescence: Complement Component C1qA Antibody (7H8) [NB200-539] - staining C1q (green) in human SHSY5Y cells by ICC/IF (Immunocytochemistry/immunofluorescence).Applications for Complement Component C1qA Antibody (7H8)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10
Application Notes
IHC-Fr: 4% PFA fixed and sucrose infiltrated frozen tissue. 18h 4C, 0.133 ug/ml 7H8. IA: Antibody 7H8 can be used as capture and detection antibody. To detect bound C11, 1/100 diluted 7H8 was used. Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
0.02% Sodium Azide
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Complement Component C1qA
Long Name
Complement Component 1q Subcomponent, A Chain
Alternate Names
C1QA
Entrez Gene IDs
12259 (Mouse)
Gene Symbol
C1QA
Additional Complement Component C1qA Products
Product Documents for Complement Component C1qA Antibody (7H8)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Complement Component C1qA Antibody (7H8)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
Citations for Complement Component C1qA Antibody (7H8)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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