Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # P12/1

Format

BSA Free
View all Complement Component C5aR1 Primary Antibodies »

Product Specifications

Immunogen

C5aR - peptide: Met1 - Asn31

Reactivity Notes

Predicted cross-reactivities: Rhesus Monkey

Localization

Multi pass membrane protein.

Specificity

Recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone P12/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31). Clone P12/1 does not inhibit the binding of C5a to its receptor.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Description

Novus Biologicals Mouse Complement Component C5aR1 Antibody (P12/1) - BSA Free (NB100-66539) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Complement Component C5aR1 Antibody (P12/1) - BSA Free

Flow Cytometry: Complement Component C5aR1 Antibody (P12/1) - BSA Free [NB100-66539]

Flow Cytometry: Complement Component C5aR1 Antibody (P12/1) - BSA Free [NB100-66539]

Flow Cytometry: Complement Component C5a R1 Antibody (P12/1) [NB100-66539] - Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD88 (NB100-66539).
Flow Cytometry: Complement Component C5aR1 Antibody (P12/1) - BSA Free [NB100-66539]

Flow Cytometry: Complement Component C5aR1 Antibody (P12/1) - BSA Free [NB100-66539]

Flow Cytometry: Complement Component C5a R1 Antibody (P12/1) [NB100-66539] - Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD88: FITC.

Applications for Complement Component C5aR1 Antibody (P12/1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:50-1:100

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement Component C5aR1

The human anaphylatoxin C5a (CD88) is a 74 residue glycopeptide which is generated by proteolytic cleavage of the complement factor C5 in the course of complement activation. A variety of biological effects evoked by C5a can be demonstrated, rendering this molecule an important mediartor of inflammation, with granulocytes and macrophages as the main target cells. All cellular responses to C5a are specifically mediated by interactions with the membrane bound C5a receptor, a seven transmembrane GTP binding coupled receptor that belongs to the rhodopsin supergene family. C5a receptor 1 expression has been reported in myeloid blood cells, brain, liver, lung, spleen, heart, kidney, and intestinal tract. ESTs have been isolated from B cell/lung/testis, nerve, and placenta libraries.

Long Name

Complement Component C5a Receptor 1

Alternate Names

C5AR1, CD88, Complement Component C5a R1

Entrez Gene IDs

728 (Human)

Gene Symbol

C5AR1

UniProt

P21730

Additional Complement Component C5aR1 Products

Product Documents for Complement Component C5aR1 Antibody (P12/1) - BSA Free

Certificate of Analysis

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Product Specific Notices for Complement Component C5aR1 Antibody (P12/1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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