Complement Factor B Antibody (014III-33.2.4.3) - (Ba fragment) - BSA Free

Novus Biologicals | Catalog # NB100-64343

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, ELISA, Flow Cytometry, Functional

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 014III-33.2.4.3

Format

BSA Free
View all Complement Factor B Primary Antibodies »

Product Specifications

Immunogen

Purified human complement factor b

Specificity

NB100-64343 recognizes the 30kDa Ba fragment of 90kDa human complement factor B, present in blood serum. C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes. NB100-64343 inhibits the function of factor B and binds both nascent factor B and the Ba cleavage product. Removal of Sodium Azide is recommended prior to use in functional assays.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Complement Factor B Antibody (014III-33.2.4.3) - (Ba fragment) - BSA Free (NB100-64343) is a monoclonal antibody validated for use in IHC, WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications

Application
Recommended Usage

ELISA

1:100-1:2000

Flow Cytometry

1:10-1:1000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
Cone 014III33.2.4.3 binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

Borate buffered saline

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Complement Factor B

This gene encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. The polyadenylation site of this gene is 421 bp from the 5' end of the gene for complement component 2.

Alternate Names

BF, BFD, C3/C5 convertase, CFAB, CFB, GBG, H2-Bf, PBF2

Entrez Gene IDs

629 (Human)

Gene Symbol

CFB

UniProt

P00751

Additional Complement Factor B Products

Product Documents

Certificate of Analysis

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Product Specific Notices

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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