Connexin 40/GJA5 Antibody

Novus Biologicals | Catalog # NBP1-46568

Novus Biologicals
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Connexin 40/GJA5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide from mouse Connexin 40/GJA5 conjugated to an blue carrier protein was used as the antigen. The peptide shares 95% identity with rat sequence.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Connexin 40/GJA5 Antibody

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.
Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568]

Immunohistochemistry-Paraffin: Connexin 40/GJA5 Antibody [NBP1-46568] - IHC on paraffin sections of rat kidney tissue using Rabbit antibody to Connexin 40. HIER: 1 mM EDTA, pH 8 for 20 min. Blocking: 0.2% LFDM in TBST filtered thru 0.2 um. Detection was done using Novolink HRP polymer from Leica following manufactures instructions. Primary antibody: dilution 1: 1000, incubated 30 min at RT (using Autostainer. Sections were counterstained with Harris Hematoxylin.

Applications for Connexin 40/GJA5 Antibody

Application
Recommended Usage

Immunohistochemistry

1:300-1:2000

Immunohistochemistry-Paraffin

1:300-1:2000

Western Blot

1:300-1:2000
Application Notes
Western Blot. A dilution of 1 : 300 to 1 : 2000 is recommended. The optimal dilution should be determined by the end user. Although not tested this antibody may work in Immunohistochemistry-Paraffin/Frozen.

Formulation, Preparation, and Storage

Purification

Unpurified

Reconstitution

Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Formulation

Lyophilized from whole antisera

Preservative

No Preservative

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Connexin 40/GJA5

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. A connexon is composed of a hexamer of connexins.

Alternate Names

ATFB11, CX40, GJA5

Entrez Gene IDs

2702 (Human)

Gene Symbol

GJA5

Additional Connexin 40/GJA5 Products

Product Documents for Connexin 40/GJA5 Antibody

Certificate of Analysis

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Product Specific Notices for Connexin 40/GJA5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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