COP1 Antibody (1E4) - Azide and BSA Free

Novus Biologicals | Catalog # H00064326-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1E4

Format

Azide and BSA Free
View all COP1 Primary Antibodies »

Product Specifications

Immunogen

RFWD2 (NP_071902, 632 a.a. ~ 731 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. CLRSFKGHINEKNFVGLASNGDYIACGSENNSLYLYYKGLSKTLLTFKFDTVKSVLDKDRKEDDTNEFVSAVCWRALPDGESNVLIAANSQGTIKVLELV

Specificity

RFWD2 - ring finger and WD repeat domain 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for COP1 Antibody (1E4) - Azide and BSA Free

Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4 Analysis of RFWD2 expression in NIH/3T3.
Immunocytochemistry/ Immunofluorescence: COP1 Antibody (1E4) [H00064326-M01]

Immunocytochemistry/ Immunofluorescence: COP1 Antibody (1E4) [H00064326-M01]

Immunocytochemistry/Immunofluorescence: COP1 Antibody (1E4) [H00064326-M01] - Analysis of monoclonal antibody to RFWD2 on NIH/3T3 cell. Antibody concentration 10 ug/ml.
Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in A-431.
Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in Jurkat.
Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01]

Western Blot: COP1 Antibody (1E4) [H00064326-M01] - RFWD2 monoclonal antibody (M01), clone 1E4. Analysis of RFWD2 expression in K-562.
Sandwich ELISA: COP1 Antibody (1E4) [H00064326-M01] - Detection limit for recombinant GST tagged RFWD2 is approximately 0.1ng/ml as a capture antibody.

Applications for COP1 Antibody (1E4) - Azide and BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COP1

COP1 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. It is directly involved in p53 ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. It ubiquitinates p53 independently of MDM2 or RCHY1. It probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, where the ubiquitin ligase activity is mediated by RBX1.

Alternate Names

Constitutive photomorphogenesis protein 1 homolog, constitutive photomorphogenic protein (COP1), COP1RNF200FLJ10416, E3 ubiquitin-protein ligase RFWD2, EC 6.3.2.-, hCOP1, putative ubiquitin ligase COP1, ring finger and WD repeat domain 2, RING finger and WD repeat domain protein 2, RING finger protein 200

Entrez Gene IDs

64326 (Human)

Gene Symbol

COP1

OMIM

608067 (Human)

UniProt

NP_071902 (Human)

Additional COP1 Products

Product Documents for COP1 Antibody (1E4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for COP1 Antibody (1E4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for COP1 Antibody (1E4) - Azide and BSA Free

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  • Q: Could you let us know whether this antibody has been mentioned in a publication so that we can look at those images? Moreover, could you please tell us whether the immunoblot presented on you website shows the labeling of the endogenous RFWD2 from 3T3 cells or whether a lysate of cells transfected with an RFWD2 expression vector has been used for blotting?

    A: This product has not yet been mentioned in any publications that we are aware of. And in regards to the WB image, this image was generated with 3T3 cells showing the staining of endogenous COP1 / RFWD2.

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