COPB2 Antibody (M3A5)

Novus Biologicals | Catalog # NB600-102

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat, Avian, Bovine, Canine, Chicken, Hamster, Monkey, Primate, Rabbit

Cited:

Rat, Bovine

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Immunoaffinity Purification

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # M3A5
View all COPB2 Primary Antibodies »

Product Specifications

Immunogen

Microtubule-associated protein from goose brain.

Reactivity Notes

kangaroo rat, goose

Localization

The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.

Specificity

Recognizes an epitope shared by the beta-COP protein (110kD) found in most tissue culture cells, and a high MW doublet of brain microtubule-associated protein (MAP 2, 270-300 kD).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for COPB2 Antibody (M3A5)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:50

Immunoprecipitation

1:10-1:500
Application Notes
AF: Use at an assay dependant dilution. ICC: Use at an assay dependant dilution. IF: CHO cells IP: Use at an assay dependant dilution. Stacked Golgi membranes from rat liver extract. Stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of the Golgi complex and a population of vesicles scattered throughout the cytoplasm.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Preservative

0.09% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: COPB2

May be used for studies on the effects of various agents influencing energy status, on disrupting the Golgi complex, or on altering the activity of G-proteins or small GTP-binding proteins on the cellular localization of beta-COP, and for the immunoaffinity purification of the beta-COP protein.

Alternate Names

beta prime subunit, Beta'-coat protein, Beta'-COP, betaprime-COP, coatomer protein complex, subunit beta 2 (beta prime), coatomer subunit beta', p102

Entrez Gene IDs

9276 (Human); 60384 (Rat)

Gene Symbol

COPB2

Additional COPB2 Products

Product Documents for COPB2 Antibody (M3A5)

Certificate of Analysis

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Product Specific Notices for COPB2 Antibody (M3A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for COPB2 Antibody (M3A5)

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Protocols

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FAQs

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