Cortisol Antibody (CORT-1) - BSA Free

Novus Biologicals | Catalog # NB200-428

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CORT-1

Format

BSA Free
View all Cortisol Primary Antibodies »

Product Specifications

Immunogen

Hybridoma clone has been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with purified cortisol conjugated with BSA.

Localization

Secreted.

Specificity

Cortisol-BSA conjugate and free cortisol. There is no cross-reactivity with BSA. Cross-reactivity, % 50% displacement of cortisol-3-125-Iodine: Cortisol (100)%; Corticosterone (20%); 17-Hydroxyprogesterone (0%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Cortisol Antibody (CORT-1) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000
Application Notes
Detection of cortisol in competitive assay.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Cortisol

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Alternate Names

Compound F, Hydrocortisone

Additional Cortisol Products

Product Documents for Cortisol Antibody (CORT-1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Cortisol Antibody (CORT-1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for Cortisol Antibody (CORT-1) - BSA Free

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  • Q: Has your Cortisol monoclonal antibody (NB200-428) been tested for cross-reactivity against the following list of compounds? Corticosterone, Prednisone, Progesterone, Cortisone, 11-deoxycortisol, Prednisolone, Testosterone, Other.

    A: We have tested NB200-428 against the following: 11-Deoxycortisol (N/A), Prednisolone (N/A), Corticosterone (20%), 11-Deoxycorticosterone (N/A), Progesterone (N/A), 17-Hydroxyprogesterone (0%), Testosterone, Estradiol, Estriol (N/A), Danazol (N/A).

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