Human Cortisol ELISA Kit (Colorimetric)

Key Product Details

Product Specifications

Precision

Intra-Assay Precision (Precision within an assay) CV% 8.27%

Inter-Assay Precision (Precision between assays) CV% 8.33%

Kit Contents for Human Cortisol ELISA Kit (Colorimetric)

• Control 1 & Control 2: For control values and ranges please refer to vial label; ready to use.
• Enzyme conjugate: Cortisol conjugated to horseradish peroxidase; ready to use.
• Microtiterwells: 12x8 (break apart) strips; wells coated with an anti-cortisol antibody (polyclonal).
• Standard A (Standard 0)*, 0 ng/mL, ready to use.
• Standard B (Standard 1)*, 0.1 ng/mL, ready to use.
• Standard C (Standard 2)*, 0.4 ng/mL, ready to use.
• Standard D (Standard 3)*, 1.7 ng/mL, ready to use.
• Standard E (Standard 4)*, 7 ng/mL, ready to use.
• Standard F (Standard 5)*, 30 ng/mL, ready to use.
• Stop Solution: Contains 2 N acidic solution; ready to use. Avoid contact with Stop Solution. It may cause skin irritations and burns.
• Substrate Solution: Contains Tetramethylbenzidine (TMB); ready to use.
• Wash Solution (10x concentrated)

Preparation and Storage

Background: Cortisol

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Additional Cortisol Products

• All Products for Cortisol
• Cortisol ELISA Kits
• Cortisol Primary Antibodies

Product Documents for Human Cortisol ELISA Kit (Colorimetric)