Fish Cortisol ELISA Kit (Colorimetric)

Novus Biologicals | Catalog # NBP3-21149

Novus Biologicals
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Key Product Details

Sample Type & Volume Required Per Well

Serum, plasma and tissue homogenates (50 - 100 uL)

Sensitivity

1.56 ng/mL

Assay Range

3.12 ng/mL - 200 ng/mL
View all Cortisol ELISA Kits »

Product Specifications

Assay Type

Competitive ELISA

Kit Type

ELISA Kit (Colorimetric)

Species

Fish

Specificity

This assay has high sensitivity and excellent specificity for detection of fish Cortisol. No significant cross-reactivity or interference between fish Cortisol and analogues was observed.

Description

Assay Length: 1-5 hours

Precision

Intra-Assay Precision (Precision within an assay) %CV 8

Inter-Assay Precision (Precision between assays) %CV 10

Recovery for Fish Cortisol ELISA Kit (Colorimetric)

Recovery

87 - 105%

Scientific Data Images for Fish Cortisol ELISA Kit (Colorimetric)

Fish Cortisol ELISA Kit (Colorimetric)

Fish Cortisol ELISA Kit (Colorimetric) [NBP3-21149] -

Fish Cortisol ELISA Kit (Colorimetric) [NBP3-21149] - Standard Curve Reference

Kit Contents for Fish Cortisol ELISA Kit (Colorimetric)

  • Adhesive Strip (For 96 wells)
  • Antibody (100 x concentrate)
  • Antibody Diluent
  • Assay plate
  • HRP-conjugate (100 x concentrate)
  • HRP-conjugate Diluent
  • Instruction manual
  • Sample Diluent
  • Standard (Freeze dried)
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (25 x concentrate)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Storage of components varies. See protocol for specific instructions.

Background: Cortisol

Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

Alternate Names

Compound F, Hydrocortisone

Additional Cortisol Products

Product Documents for Fish Cortisol ELISA Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Fish Cortisol ELISA Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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