COUP-TF I/NR2F1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-31259

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry-Frozen, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all COUP-TF I/NR2F1 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human COUP-TF I/NR2F1. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

46 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COUP-TF I/NR2F1 Antibody - BSA Free

Western Blot: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Western Blot: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Western Blot: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with COUP TF1 antibody [N1], N-term diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Immunocytochemistry/ Immunofluorescence: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Immunocytochemistry/Immunofluorescence: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Analysis of HeLa, using COUP TF1 antibody at 1:200 dilution.
Immunohistochemistry-Frozen: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Immunohistochemistry-Frozen: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Immunohistochemistry-Frozen: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Analysis of embryonic mouse brain tissue using COUP-TF I/NR2F1 antibody. Primary antibody dilution:1:200. Citrate retrieval done 30um thick sections. Image from verified customer review.
Immunohistochemistry-Paraffin: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Immunohistochemistry-Paraffin: COUP-TF I/NR2F1 Antibody [NBP1-31259]

Immunohistochemistry-Paraffin: COUP-TF I/NR2F1 Antibody [NBP1-31259] - Mouse hind brain. COUP TF1 antibody [N1], N-term diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for COUP-TF I/NR2F1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Frozen

Reported in scientific literature (PMID:31996853).

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Reviewed Applications

Read 1 review rated 5 using NBP1-31259 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 10% Glycerol

Format

BSA Free

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: COUP-TF I/NR2F1

Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. It has varied roles in the development of the peripheral nervous system, such as early regionalization of the neocortex, differentiation of subplate neurons and guidance of thalamocortical axons.

Long Name

COUP Transcription Factor 1/Nuclear Receptor Subfamily 2, Group F, Member 1

Alternate Names

COUPTF I, ERBAL3, NR2F1

Gene Symbol

NR2F1

Additional COUP-TF I/NR2F1 Products

Product Documents for COUP-TF I/NR2F1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for COUP-TF I/NR2F1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Citations for COUP-TF I/NR2F1 Antibody - BSA Free

Customer Reviews for COUP-TF I/NR2F1 Antibody - BSA Free (1)

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Customer Images

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  • COUP-TF I/NR2F1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: Brain (cerebral cortex)
    Species: Mouse
    Verified Customer | Posted 04/07/2022
    Embryonic brain couptf1/NR2F1 staining
    Embryonic brain couptf1/NR2F1 staining 1:200 Citrate retrieval done 30um thick sections
    COUP-TF I/NR2F1 Antibody - BSA Free NBP1-31259

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Protocols

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FAQs

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