COX4 Antibody (6B3)

Novus Biologicals | Catalog # NBP1-51650

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6B3
View all COX4 Primary Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human COX4 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Marker

Mitochondria Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

19 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for COX4 Antibody (6B3)

Western Blot: COX4 Antibody (6B3)BSA Free [NBP1-51650]

Western Blot: COX4 Antibody (6B3)BSA Free [NBP1-51650]

Western Blot: COX4 Antibody (6B3) [NBP1-51650] - Analysis using COX4I1 mouse mAb against HEK293 (1), A549 (2) and PC12 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: COX4 Antibody (6B3) - BSA Free [NBP1-51650]

Immunocytochemistry/ Immunofluorescence: COX4 Antibody (6B3) - BSA Free [NBP1-51650]

Immunocytochemistry/Immunofluorescence: COX4 Antibody (6B3) [NBP1-51650] - Analysis of PANC-1 cells using COX4I1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Flow Cytometry: COX4 Antibody (6B3) - BSA Free [NBP1-51650]

Flow Cytometry: COX4 Antibody (6B3) - BSA Free [NBP1-51650]

Flow Cytometry: COX4 Antibody (6B3) [NBP1-51650] - Analysis of K562 cells using COX4I1 mouse mAb (blue) and negative control (red).

Applications for COX4 Antibody (6B3)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Flow Cytometry Panel Builder

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Ascites

Formulation

Ascites

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: COX4

Cytochrome c oxidase (COX) is the terminal enzyme of the mitochondrial respiratory chain. It is a multi-subunit enzyme complex that couples the transfer of electrons from cytochrome c to molecular oxygen and contributes to a proton electrochemical gradient across the inner mitochondrial membrane. The complex consists of 13 mitochondrial- and nuclear-encoded subunits. The mitochondrially-encoded subunits perform the electron transfer and proton pumping activities. The functions of the nuclear-encoded subunits are unknown but they may play a role in the regulation and assembly of the complex. This gene encodes the nuclear-encoded subunit IV isoform 1 of the human mitochondrial respiratory chain enzyme. It is located at the 3' of the NOC4 (neighbor of COX4) gene in a head-to-head orientation, and shares a promoter with it.

Long Name

Cytochrome c Oxidase Subunit IV

Alternate Names

COX4I2

Entrez Gene IDs

1327 (Human); 12857 (Mouse); 29445 (Rat)

Gene Symbol

COX4I1

UniProt

P13073 (Human)

Additional COX4 Products

Product Documents for COX4 Antibody (6B3)

Certificate of Analysis

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Download SDS

Product Specific Notices for COX4 Antibody (6B3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for COX4 Antibody (6B3)

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Protocols

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FAQs

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