COX5b Antibody - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-97091
![Western Blot: COX5b Antibody [NBP2-97091]](https://resources.rndsystems.com/images/products/COX5b-Antibody-Western-Blot-NBP2-97091-img0006.jpg "Western Blot: COX5b Antibody [NBP2-97091]")
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
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Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human COX5b (Accession#: P10606; Ala32-His129)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for COX5b Antibody - Azide and BSA Free
Western Blot: COX5b Antibody [NBP2-97091]
Western Blot: COX5b Antibody [NBP2-97091] - Anti-COX5b rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 13 kDa![Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091]](https://resources.rndsystems.com/images/products/COX5b-Antibody-Immunohistochemistry-Paraffin-NBP2-97091-img0004.jpg "Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091]")
Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091]
Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091] - Immunochemical staining of human COX5b in human liver with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).![Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091]](https://resources.rndsystems.com/images/products/COX5b-Antibody-Immunohistochemistry-Paraffin-NBP2-97091-img0003.jpg "Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091]")
Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091]
Immunohistochemistry-Paraffin: COX5b Antibody [NBP2-97091] - Immunochemical staining of human COX5b in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).![Immunoprecipitation: COX5b Antibody [NBP2-97091]](https://resources.rndsystems.com/images/products/COX5b-Antibody-Immunoprecipitation-NBP2-97091-img0005.jpg "Immunoprecipitation: COX5b Antibody [NBP2-97091]")
Immunoprecipitation: COX5b Antibody [NBP2-97091]
Immunoprecipitation: COX5b Antibody [NBP2-97091] - COX5b was immunoprecipitated using: Lane A: 0.5 mg HepG2 Whole Cell Lysate 2 ul anti-COX5b rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-COX5b rabbit polyclonal antibody, at 1:200 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 14 kDaApplications for COX5b Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:5000-1:10000
Immunohistochemistry-Paraffin
1:1000-1:4000
Immunoprecipitation
1-4 uL/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: COX5b
Alternate Names
COXVB, Cytochrome c oxidase polypeptide Vb, cytochrome c oxidase polypeptide VB, mitochondrial, cytochrome c oxidase subunit 5B, mitochondrial, cytochrome c oxidase subunit Vb
Gene Symbol
COX5B
Additional COX5b Products
Product Documents for COX5b Antibody - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for COX5b Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- Detection & Visualization of Antibody Binding
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- R&D Systems Quality Control Western Blot Protocol
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: ELISA
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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