CPS1 Antibody (1R6W3)

Novus Biologicals | Catalog # NBP3-16357

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1R6W3 expressed in HEK293
View all CPS1 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 100-200 of human CPS1 (NP_001866.2). IIGNGGAPDTTALDELGLSKYLESNGIKVSGLLVLDYSKDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQPVDFVDPNKQNL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for CPS1 Antibody (1R6W3)

Western Blot: CPS1 Antibody (1R6W3) [NBP3-16357]

Western Blot: CPS1 Antibody (1R6W3) [NBP3-16357]

Western Blot: CPS1 Antibody (1R6W3) [NBP3-16357] - Analysis of extracts of various cell lines, using CPS1 Rabbit mAb (NBP3-16357) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: CPS1 Antibody (1R6W3) [NBP3-16357]

Immunohistochemistry: CPS1 Antibody (1R6W3) [NBP3-16357]

Immunohistochemistry: CPS1 Antibody (1R6W3) [NBP3-16357] - Immunofluorescence analysis of rat liver using CPS1 Rabbit mAb (NBP3-16357) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: CPS1 Antibody (1R6W3) [NBP3-16357]

Immunohistochemistry-Paraffin: CPS1 Antibody (1R6W3) [NBP3-16357]

Immunohistochemistry-Paraffin: CPS1 Antibody (1R6W3) [NBP3-16357] - Human liver using CPS1 Rabbit mAb (NBP3-16357) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
CPS1 Antibody (1R6W3)

Immunocytochemistry/ Immunofluorescence: CPS1 Antibody (1R6W3) [NBP3-16357] -

Immunocytochemistry/ Immunofluorescence: CPS1 Antibody (1R6W3) [NBP3-16357] - Confocal imaging of paraffin-embedded Human liver using CPS1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
CPS1 Antibody (1R6W3)

Immunocytochemistry/ Immunofluorescence: CPS1 Antibody (1R6W3) [NBP3-16357] -

Immunocytochemistry/ Immunofluorescence: CPS1 Antibody (1R6W3) [NBP3-16357] - Confocal imaging of paraffin-embedded Rat liver using CPS1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Applications for CPS1 Antibody (1R6W3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: CPS1

The protein encoded by the CPS1 gene is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. There are two isozymes of this enzyme, and the encoded protein is the mitochondrial form. Three transcript variants encoding different isoforms have been found for this gene. The shortest isoform may not be localized to the mitochondrion.

Alternate Names

carbamoyl-phosphate synthase [ammonia], mitochondrial, carbamoyl-phosphate synthase 1, mitochondrial, carbamoyl-phosphate synthetase 1, mitochondrial, carbamoylphosphate synthetase I, Carbamoyl-phosphate synthetase I, CPSase I, CPSASE1, EC 6.3.4.16

Gene Symbol

CPS1

Additional CPS1 Products

Product Documents for CPS1 Antibody (1R6W3)

Certificate of Analysis

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Product Specific Notices for CPS1 Antibody (1R6W3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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