![Western Blot: CPSF1 Antibody [NB100-79822]](https://resources.rndsystems.com/images/products/CPSF1-Antibody-Western-Blot-NB100-79822-img0008.jpg "Western Blot: CPSF1 Antibody [NB100-79822]")
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Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
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Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1392 and 1442 of human cleavage and polyadenylation specific factor 1, 160kD subunit using the numbering given in entry NP_037423.1 (GeneID 29894).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for CPSF1 Antibody
Western Blot: CPSF1 Antibody [NB100-79822]
Western Blot: CPSF1 Antibody [NB100-79822] - Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-CPSF160 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescencewith an exposure time of 30 seconds.![Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]](https://resources.rndsystems.com/images/products/CPSF1-Antibody-Immunohistochemistry-NB100-79822-img0004.jpg "Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]")
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB![Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]](https://resources.rndsystems.com/images/products/CPSF1-Antibody-Immunohistochemistry-NB100-79822-img0005.jpg "Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]")
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822]
Immunohistochemistry-Paraffin: CPSF1 Antibody [NB100-79822] - Section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-CPSF160 (NB100-79822). Detection: DAB![Immunoprecipitation: CPSF1 Antibody [NB100-79822]](https://resources.rndsystems.com/images/products/CPSF1-Antibody-Immunoprecipitation-NB100-79822-img0009.jpg "Immunoprecipitation: CPSF1 Antibody [NB100-79822]")
Immunoprecipitation: CPSF1 Antibody [NB100-79822]
Immunoprecipitation: CPSF1 Antibody [NB100-79822] - Detection of human CPSF160 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-CPSF160 antibody NB100-79822 (lot NB100-79822-2) used for IP at 6 ug per reaction. CPSF160 was also immunoprecipitated by a previous lot of this antibody (lot NB100-79822-1). For blotting immunoprecipitated CPSF160, NB100-79822 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for CPSF1 Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: CPSF1
Alternate Names
cleavage and polyadenylation specific factor 1, 160kD subunit, cleavage and polyadenylation specific factor 1, 160kDa, Cleavage and polyadenylation specificity factor 160 kDa subunit, cleavage and polyadenylation specificity factor subunit 1, CPSF 160 kDa subunit, CPSF160, HSU37012, P/cl.18, polyadenylation specificity factor
Gene Symbol
CPSF1
UniProt
Additional CPSF1 Products
Product Documents for CPSF1 Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for CPSF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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